Mouse N-Cadherin/Cadherin-2/CD325 (NCAD) Monoclonal Antibody | anti-NCAD antibody

N-Cadherin/Cadherin-2/CD325 (NCAD)

Cat. Num. AAA62743

• Gene Names: CDH2; CDHN; NCAD; CD325; CDw325
• Reactivity: Human, Mouse. Others not known.
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: N-Cadherin/Cadherin-2/CD325 (NCAD), Antibody; N-Cadherin/Cadherin-2/CD325 (NCAD); Cadherin-2 N cadherin neuronal; Cadherin-2 type 1; Cadherin-2; Calcium dependent adhesion protein neuronal; CD325; CDH2; CDHN; CDw325; N-Cadherin; NCAD; anti-NCAD antibody

• Host: Mouse
• Reactivity: Human, Mouse. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: CDH2/1573
• Specificity: Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. Expression of N-cadherin has been reported on a variety of normal tissues including neuronal, endothelial and muscle cells, and a subpopulation of early hematopoietic progenitor cells. Results aid in the classification of malignant non-carcinomatous neoplasms including mesotheliomas, chordomas, synovial sarcomas, malignant melanomas, epithelioid sarcomas, epithelioid angiosarcomas, clear cell sarcomas as well as serous and endometrioid tumors of the ovary have been demonstrated to be N-cadherin positive, whereas mucinous tumors are negative. Other N-cadherin-positive neoplasms include renal cell carcinomas and some variant breast tumors, including medullary breast carcinomas and sarcomatoid metaplastic breast carcinomas.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 875

• Applicable Applications for anti-NCAD antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant human CDH2 intracellular domain
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 18q11.2
• Positive Control: HeLa or HUVEC cells. Heart, Pancreas or Cerebral Cortex
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis of Purified Chromogranin A Mouse Recombinant Monoclonal Antibody (rCHGA/798).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded Mouse Heart stained with N-Cadherin Monoclonal Antibody (CDH2/1573).)

References

Han AC, Peralta Soler A, Knudsen KA, Wheelock MJ, Johnson KR, Salazar H. Differential expression of N-cadherin in pleural mesotheliomas and E-cadherin in lung adenocarcinomas in formalin-fixed, paraffin-embedded tissues. Hum Pathol. 1997; 28(6):641

NCBI and Uniprot Product Information

• NCBI GI #: 815890961
• NCBI GeneID: 1000
• NCBI Accession #: NP_001295105.1
• NCBI GenBank Nucleotide #: NM_001308176.1
• UniProt Accession #: P19022; Q14923; Q8N173; A8MWK3; B0YIY6
• Molecular Weight: 130-140kDa
• NCBI Official Full Name: cadherin-2 isoform 2
• NCBI Official Synonym Full Names: cadherin 2
• NCBI Official Symbol: CDH2
• NCBI Official Synonym Symbols: CDHN; NCAD; CD325; CDw325
• NCBI Protein Information: cadherin-2
• UniProt Protein Name: Cadherin-2
• UniProt Gene Name: CDH2
• UniProt Synonym Gene Names: CDHN; NCAD; N-cadherin