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WB (Western Blot)
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NAMPT/PBEF/Visfatin Recombinant Antibody | NAMPT/PBEF recombinant antibody

Rabbit anti-NAMPT/PBEF/Visfatin Recombinant Monoclonal Antibody [BLR058F]

Gene Names
NAMPT; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
NAMPT/PBEF/Visfatin, Recombinant Antibody; Rabbit anti-NAMPT/PBEF/Visfatin Recombinant Monoclonal Antibody [BLR058F]; PBEF1; pre-B cell-enhancing factor; pre-B-cell colony-enhancing factor 1; VISFATIN; nicotinamide phosphoribosyltransferase; Nampt; NAmPRTase; 1110035O14Rik; VF; PBEF; Nicotinamide phosphoribosyltransferase; NAMPT/PBEF recombinant antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR058F
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for NAMPT/PBEF recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 5 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Immunogen
Between 400 and 450
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human and mouse NAMPT/PBEF/Visfatin by western blot. Samples: Whole cell lysate (50 ug) from RKO, MCF-7, K-562, GaMG, HEK293T, HeLa, U2OS, LNCaP, Hep-G2, Jurkat, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody (AAA23815 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-GAPDH .)

IP (Immunoprecipitation)

(Detection of human NAMPT/PBEF/Visfatin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody (AAA23815 lot 1) used for IP at 5 ul per reaction. NAMPT/PBEF/Visfatin was also immunoprecipitated by rabbit anti-NAMPT/PBEF/Visfatin antibodies . For blotting immunoprecipitated NAMPT/PBEF/Visfatin, AAA23815 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)

IHC (Immunohistochemistry)

(Detection of human NAMPT/PBEF/Visfatin by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody (AAA23815 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human NAMPT/PBEF/Visfatin by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody (AAA23815 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human NAMPT/PBEF/Visfatin by immunocytochemistry. Sample: FFPE section of NCI-H460. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody (AAA23815 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human NAMPT/PBEF/Visfatin (shaded) in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal (AAA23815 lot 1) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Related Product Information for NAMPT/PBEF recombinant antibody
PBEF is also known as visfatin and was first identified as a factor that enhanced the differentiation of B-cells. It is a secreted factor that is highly expressed in adipocytes. Intracellularly, PBEF acts as a cytosolic enzyme involved in nicotinamide adenine dinucleotide (NAD) synthesis. PBEF levels positively correlate with obesity and type 2 diabetes mellitus and can bind the insulin receptor to lower blood sugar.
Product Categories/Family for NAMPT/PBEF recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
nicotinamide phosphoribosyltransferase
NCBI Official Synonym Full Names
nicotinamide phosphoribosyltransferase
NCBI Official Symbol
NAMPT
NCBI Official Synonym Symbols
VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
NCBI Protein Information
nicotinamide phosphoribosyltransferase; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1
UniProt Protein Name
Nicotinamide phosphoribosyltransferase
UniProt Gene Name
NAMPT
UniProt Synonym Gene Names
PBEF; PBEF1; NAmPRTase; Nampt; Pre-B cell-enhancing factor
UniProt Entry Name
NAMPT_HUMAN

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Product Notes

The NAMPT/PBEF nampt (Catalog #AAA23815) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NAMPT/PBEF/Visfatin Recombinant Monoclonal Antibody [BLR058F] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NAMPT/PBEF/Visfatin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 5 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the NAMPT/PBEF nampt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAMPT/PBEF/Visfatin, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.