NAMPT/PBEF/Visfatin Recombinant Antibody | NAMPT/PBEF recombinant antibody

Rabbit anti-NAMPT/PBEF/Visfatin Recombinant Monoclonal Antibody [BLR058F]

Cat. Num. AAA23815

• Gene Names: NAMPT; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Purified
• Synonyms: NAMPT/PBEF/Visfatin; Monoclonal Recombinant Antibody; Rabbit anti-NAMPT/PBEF/Visfatin Recombinant Monoclonal Antibody [BLR058F]; PBEF1; pre-B cell-enhancing factor; pre-B-cell colony-enhancing factor 1; VISFATIN; nicotinamide phosphoribosyltransferase; Nampt; NAmPRTase; 1110035O14Rik; VF; PBEF; Nicotinamide phosphoribosyltransferase; NAMPT/PBEF recombinant antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: BLR058F
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 250ug/ml (varies by lot)

• Applicable Applications for NAMPT/PBEF recombinant antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IP: 5 ul/mg lysate; IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
• Immunogen: Between 400 and 450
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human and mouse NAMPT/PBEF/Visfatin by western blot. Samples: Whole cell lysate (50 ug) from RKO, MCF-7, K-562, GaMG, HEK293T, HeLa, U2OS, LNCaP, Hep-G2, Jurkat, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody (AAA23815 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-GAPDH .)

IP (Immunoprecipitation)

(Detection of human NAMPT/PBEF/Visfatin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody (AAA23815 lot 1) used for IP at 5 ul per reaction. NAMPT/PBEF/Visfatin was also immunoprecipitated by rabbit anti-NAMPT/PBEF/Visfatin antibodies . For blotting immunoprecipitated NAMPT/PBEF/Visfatin, AAA23815 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)

IHC (Immunohistochemistry)

(Detection of human NAMPT/PBEF/Visfatin by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody (AAA23815 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry)

(Detection of human NAMPT/PBEF/Visfatin by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody (AAA23815 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human NAMPT/PBEF/Visfatin by immunocytochemistry. Sample: FFPE section of NCI-H460. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal antibody (AAA23815 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human NAMPT/PBEF/Visfatin (shaded) in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-NAMPT/PBEF/Visfatin recombinant monoclonal (AAA23815 lot 1) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Related Product Information for NAMPT/PBEF recombinant antibody

PBEF is also known as visfatin and was first identified as a factor that enhanced the differentiation of B-cells. It is a secreted factor that is highly expressed in adipocytes. Intracellularly, PBEF acts as a cytosolic enzyme involved in nicotinamide adenine dinucleotide (NAD) synthesis. PBEF levels positively correlate with obesity and type 2 diabetes mellitus and can bind the insulin receptor to lower blood sugar.

Product Categories/Family for NAMPT/PBEF recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 5031977
• NCBI GeneID: 10135
• NCBI Accession #: NP_005737.1
• NCBI GenBank Nucleotide #: NM_005746.2
• UniProt Accession #: P43490; Q3KQV0; Q8WW95; A4D0Q9; A4D0R0
• NCBI Official Full Name: nicotinamide phosphoribosyltransferase
• NCBI Official Synonym Full Names: nicotinamide phosphoribosyltransferase
• NCBI Official Symbol: NAMPT
• NCBI Official Synonym Symbols: VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
• NCBI Protein Information: nicotinamide phosphoribosyltransferase; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1
• UniProt Protein Name: Nicotinamide phosphoribosyltransferase
• UniProt Gene Name: NAMPT
• UniProt Synonym Gene Names: PBEF; PBEF1; NAmPRTase; Nampt; Pre-B cell-enhancing factor
• UniProt Entry Name: NAMPT_HUMAN