Mouse NCAM / CD56 Monoclonal Antibody | anti-NCAM antibody

Mouse Monoclonal [clone MEM-188] (IgG2a) to Human NCAM / CD56

Cat. Num. AAA51785

• Gene Names: NCAM1; CD56; NCAM; MSK39
• Reactivity: Bovine, Human, Pig
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified from tissue culture supernant
• Synonyms: NCAM / CD56, Antibody; Mouse Monoclonal [clone MEM-188] (IgG2a) to Human NCAM / CD56; Anti-NCAM / CD56 Antibody (clone MEM-188) IHC-plus; NCAM1; CD56; CD56 antigen; MSK39; NCAM-1; N-CAM-1; NCAM; Human NCAM; anti-NCAM antibody

• Host: Mouse
• Reactivity: Bovine, Human, Pig
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: MEM-188
• Specificity: Human CD56
• Purity/Purification: Affinity Purified from tissue culture supernant
• Form/Format: Phosphate-buffered solution, pH 7.2, 0.09% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 848

• Applicable Applications for anti-NCAM antibody: FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IHC (Immunohistochemistry)
• Target: NCAM / CD56
• Target Species: Human
• Immunogen Type: Cells.
• Immunogen: NCAM / CD56 antibody was raised against kG-1 human acute myelogenous leukima cell line
• Family/Subfamily: Immunoglobulin / Not assigned-immunoglobulin
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C.

FCM/FACS (Flow Cytometry)

(Human peripheral blood lymphocytes stained with purified MEM-188, followed by anti-mouse IgGs FITC)

IHC (Immunohistochemistry)

(Anti-CD56 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Product Categories/Family for anti-NCAM antibody

Family: Immunoglobulin

NCBI and Uniprot Product Information

• NCBI GI #: 94420689
• NCBI GeneID: 4684
• NCBI Accession #: NP_000606.3
• NCBI GenBank Nucleotide #: NM_000615.6
• UniProt Accession #: P13591; P13592; P13593; Q05C58; Q15829; Q16180; Q16209; Q59FL7; Q86X47; Q96CJ3; A8K8T8
• NCBI Official Full Name: neural cell adhesion molecule 1 isoform 1
• NCBI Official Synonym Full Names: neural cell adhesion molecule 1
• NCBI Official Symbol: NCAM1
• NCBI Official Synonym Symbols: CD56; NCAM; MSK39
• NCBI Protein Information: neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; antigen recognized by monoclonal antibody 5.1H11
• UniProt Protein Name: Neural cell adhesion molecule 1
• UniProt Gene Name: NCAM1
• UniProt Synonym Gene Names: NCAM; N-CAM-1; NCAM-1
• UniProt Entry Name: NCAM1_HUMAN