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product-image-AAA312562_ICC8.jpg ICC (Immunocytochemistry) (ICC staining NDUFS3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Rabbit anti-Human NDUFS3 Monoclonal Antibody | anti-NDUFS3 antibody

NDUFS3 Antibody

Gene Names
NDUFS3; CI-30
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
NDUFS3, Antibody; NDUFS3 Antibody; CI 30 antibody; CI 30KD antibody; CI-30kD antibody; Complex I 30KD antibody; Complex I 30kDa subunit antibody; COMPLEX I; MITOCHONDRIAL RESPIRATORY CHAIN; 30-KD SUBUNIT antibody; Complex I-30kD antibody; mitochondrial antibody; NADH coenzyme Q reductase antibody; NADH dehydrogenase (ubiquinone) Fe S protein 3 30kDa antibody; NADH dehydrogenase [ubiquinone] iron sulfur protein 3 mitochondrial antibody; NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 antibody; NADH dehydrogenase ubiquinone 30 kDa subunit antibody; NADH-ubiquinone oxidoreductase 30 kDa subunit antibody; NADH-Ubiquinone Oxidoreductase Fe-S Protein 3 antibody; NDUFS3 antibody; NDUS3_HUMAN antibody; anti-NDUFS3 antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
JU10-32
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-NDUFS3 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

ICC (Immunocytochemistry)

(ICC staining NDUFS3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

product-image-AAA312562_ICC8.jpg ICC (Immunocytochemistry) (ICC staining NDUFS3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining NDUFS3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

product-image-AAA312562_ICC10.jpg ICC (Immunocytochemistry) (ICC staining NDUFS3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemisry)

(Immunohistochemical analysis of paraffin-embedded human kidney cancer tissues using anti-NDUFS3 receptor 2 antibody. Counter stained with hematoxylin.)

product-image-AAA312562_IHC11.jpg IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human kidney cancer tissues using anti-NDUFS3 receptor 2 antibody. Counter stained with hematoxylin.)

IHC (Immunohiostchemistry)

(Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using anti-NDUFS3 receptor 2 antibody. Counter stained with hematoxylin.)

product-image-AAA312562_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using anti-NDUFS3 receptor 2 antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of NDUFS3 on HepG2 cell using anti-NDUFS3 antibody at 1/500 dilution.)

product-image-AAA312562_WB15.jpg WB (Western Blot) (Western blot analysis of NDUFS3 on HepG2 cell using anti-NDUFS3 antibody at 1/500 dilution.)
Related Product Information for anti-NDUFS3 antibody
NDUFS3 (NADH dehydrogenase ubiquinone iron-sulfur protein 3) is one of about 45 subunits comprising complex I of the oxidative phosphorylation electron transport chain. The multisubunit NADH: ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of the mitochondria. NDUFS3 is the last subunit of the seven subunits that make up the core of complex I. Through use of chaotropic agents, complex I can be separated into three different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. The IP fraction includes NDUFS1-7. NDUFS3 contains a highly conserved casein kinase II phosphorylation site. Mutations in the NDUFS3 gene may cause optic atrophy, Leigh syndrome and complex I deficiency.
Product Categories/Family for anti-NDUFS3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,242 Da
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
NCBI Official Symbol
NDUFS3
NCBI Official Synonym Symbols
CI-30
NCBI Protein Information
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial; NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial; CI-30kD; complex I-30kD; complex I 30kDa subunit; NADH dehydrogenase-ubiquinone 30 kDa subunit; NADH-ubiquinone oxi
UniProt Protein Name
NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial
UniProt Gene Name
NDUFS3
UniProt Synonym Gene Names
CI-30kD
UniProt Entry Name
NDUS3_HUMAN

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Product Notes

The NDUFS3 ndufs3 (Catalog #AAA312562) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDUFS3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFS3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NDUFS3 ndufs3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFS3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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