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WB (Western Blot)
(Detection of human Nectin-2/CD112 by western blot. Samples: Whole cell lysate (50 ug) from OVCAR-8, Jurkat, U2OS, GaMG, OVCAR-4, 786-O, A-549, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Rabbit anti-GAPDH .)
Nectin-2/CD112 Recombinant Antibody | CD112 recombinant antibody
Rabbit anti-Nectin-2/CD112 Recombinant Monoclonal Antibody [BLR071G]
Gene Names
NECTIN2; HVEB; PRR2; CD112; PVRL2; PVRR2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
Purified
Synonyms
Nectin-2/CD112; Monoclonal Recombinant Antibody; Rabbit anti-Nectin-2/CD112 Recombinant Monoclonal Antibody [BLR071G]; CD112; poliovirus receptor-like 2; PVRR2; PVRL2; PRR2; Poliovirus receptor-related protein 2; poliovirus receptor-related 2 (herpesvirus entry mediator B); nectin-2; Nectin cell adhesion molecule 2; HVEB; herpesvirus entry protein B; Herpes virus entry mediator B; CD antigen CD112; Herpesvirus entry mediator B; Nectin-2; nectin cell adhesion molecule 2; CD112 recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR071G
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for CD112 recombinant antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF)
Application Notes
WB: 1:1000
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunogen
Between 50 and 100
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Nectin-2/CD112 by western blot. Samples: Whole cell lysate (50 ug) from OVCAR-8, Jurkat, U2OS, GaMG, OVCAR-4, 786-O, A-549, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Rabbit anti-GAPDH .)
WB (Western Blot)
(Detection of human Nectin-2/CD112 by western blot. Samples: Whole cell lysate (50 ug) from OVCAR-8, Jurkat, U2OS, GaMG, OVCAR-4, 786-O, A-549, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Rabbit anti-GAPDH .)
IHC (Immunohistochemistry)
(Detection of human Nectin-2/CD112 in FFPE hepatic carcinoma by IHC-IF. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI.)
IHC (Immunohistochemistry)
(Detection of human Nectin-2/CD112 in FFPE hepatic carcinoma by IHC-IF. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI.)
IHC (Immunohistochemistry)
(Detection of human Nectin-2/CD112 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1) used at 1:200. Secondary: DyLight 594-conjugated goat anti-rabbit IgG . Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human Nectin-2/CD112 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1) used at 1:200. Secondary: DyLight 594-conjugated goat anti-rabbit IgG . Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human Nectin-2/CD112 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human Nectin-2/CD112 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human Nectin-2/CD112 by immunohistochemistry. Sample: FFPE section of prostate carcinoma. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human Nectin-2/CD112 by immunohistochemistry. Sample: FFPE section of prostate carcinoma. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human Nectin-2/CD112 by immunocytochemistry. Sample: FFPE section of OVCAR-8 cells. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human Nectin-2/CD112 by immunocytochemistry. Sample: FFPE section of OVCAR-8 cells. Antibody: Rabbit anti-Nectin-2/CD112 recombinant monoclonal antibody (AAA23819 lot 1) used at 1:200. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for CD112 recombinant antibody
Nectin-2/CD112 is a type I membrane glycoprotein of the Immunoglobulin superfamily. It is a plasma membrane components of adherens junctions and acts as a receptor for certain mutant strains of herpes simplex virus and pseudorabies virus. Nectin-2/CD112 is also a modulator of T-cell signaling. [taken from NCBI Entrez Gene (Gene ID: 389677)].
Product Categories/Family for CD112 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,359 Da
NCBI Official Full Name
nectin-2 isoform alpha
NCBI Official Synonym Full Names
nectin cell adhesion molecule 2
NCBI Official Symbol
NECTIN2
NCBI Official Synonym Symbols
HVEB; PRR2; CD112; PVRL2; PVRR2
NCBI Protein Information
nectin-2
UniProt Protein Name
Nectin-2
UniProt Gene Name
NECTIN2
UniProt Synonym Gene Names
Herpesvirus entry mediator B; HveB
Similar Products
Product Notes
The CD112 nectin2 (Catalog #AAA23819) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Nectin-2/CD112 Recombinant Monoclonal Antibody [BLR071G] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nectin-2/CD112 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF). WB: 1:1000 IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Researchers should empirically determine the suitability of the CD112 nectin2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nectin-2/CD112, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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