WB (Western Blot)
(Detection of human NEDD-9/CASL/HEF1 by western blot. Samples: Whole cell lysate (50 ug) from OVCAR-8, U-251MG, A-549, and UACC-257 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (AAA213662 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
NEDD-9/CASL/HEF1 recombinant antibody
Rabbit anti-NEDD-9/CASL/HEF1 Recombinant Monoclonal Antibody [BLR248L]
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
NEDD-9/CASL/HEF1, Recombinant Antibody; Rabbit anti-NEDD-9/CASL/HEF1 Recombinant Monoclonal Antibody [BLR248L]; CRK-associated substrate-related protein; Cas scaffolding protein family member 2; p130Cas-related protein; p105; neural precursor cell expressed developmentally down-regulated protein 9; NEDD-9; HEF1; enhancer of filamentation 1; renal carcinoma antigen NY-REN-12; Crk-associated substrate related protein Cas-L; CASS2; cas-like docking; CAS-L; CASL; CAS2; Enhancer of filamentation 1 p55; Enhancer of filamentation 1; neural precursor cell expressed, developmentally down-regulated 9; NEDD-9/CASL/HEF1 recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR248L
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for NEDD-9/CASL/HEF1 recombinant antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 150 and 200
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human NEDD-9/CASL/HEF1 by western blot. Samples: Whole cell lysate (50 ug) from OVCAR-8, U-251MG, A-549, and UACC-257 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (AAA213662 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of human NEDD-9/CASL/HEF1 by western blot. Samples: Whole cell lysate (50 ug) from OVCAR-8, U-251MG, A-549, and UACC-257 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (AAA213662 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse NEDD-9/CASL/HEF1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 and C2C12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (AAA213662 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse NEDD-9/CASL/HEF1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 and C2C12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (AAA213662 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation)
(Detection of human NEDD-9/CASL/HEF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-8 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (AAA213662 lot 1) used for IP at 6 ul/mg lysate. NEDD-9/CASL/HEF1 was also immunoprecipitated by a second antibody against a different epitope of NEDD-9/CASL/HEF1 (Antibody 2). For blotting immunoprecipitated NEDD-9/CASL/HEF1, AAA213662 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human NEDD-9/CASL/HEF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-8 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-NEDD-9/CASL/HEF1 recombinant monoclonal antibody (AAA213662 lot 1) used for IP at 6 ul/mg lysate. NEDD-9/CASL/HEF1 was also immunoprecipitated by a second antibody against a different epitope of NEDD-9/CASL/HEF1 (Antibody 2). For blotting immunoprecipitated NEDD-9/CASL/HEF1, AAA213662 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for NEDD-9/CASL/HEF1 recombinant antibody
NEDD-9, aka CASL or HEF1, is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. NEDD-9 is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. [taken from NCBI Entrez Gene (Gene ID: 4739)].
Product Categories/Family for NEDD-9/CASL/HEF1 recombinant antibody
NCBI and Uniprot Product Information
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Product Notes
The NEDD-9/CASL/HEF1 nedd9 (Catalog #AAA213662) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NEDD-9/CASL/HEF1 Recombinant Monoclonal Antibody [BLR248L] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NEDD-9/CASL/HEF1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NEDD-9/CASL/HEF1 nedd9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEDD-9/CASL/HEF1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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