IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NEK9 protein using NEK9 antibody)
Mouse NEK9 Monoclonal Antibody | anti-NEK9 antibody
NEK9 antibody
Gene Names
NEK9; Nek8; NERCC; NERCC1
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
NEK9 antibody was purified by affinity chromatography.
Synonyms
NEK9, Antibody; NEK9 antibody; Monoclonal NEK9; Anti-NEK9; Nima; Never In Mitosis Gene A-Related Kinase 9; DKFZp434D0935; MGC138306; MGC16714; NERCC; NERCC1; Nek8; NEK9; NEK 9; NEK-9; anti-NEK9 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
7G7
Purity/Purification
NEK9 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
979
Applicable Applications for anti-NEK9 antibody
WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Biological Significance
NEK9 is a pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. NEK9 phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. NEK9 phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression.
Cross-Reactivity
Human
Immunogen
NEK9 antibody was raised in mouse using a full length recombinant protein of human NEK9 (NP_149107) produced in HEK293 cell as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NEK9 protein using NEK9 antibody)
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NEK9 protein using NEK9 antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NEK9 protein (Right) or empty vector (Left) detected with NEK9 antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NEK9 protein (Right) or empty vector (Left) detected with NEK9 antibody)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant NEK9 protein (red) or empty vector (blue) stained using NEK9 antibody)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant NEK9 protein (red) or empty vector (blue) stained using NEK9 antibody)
Related Product Information for anti-NEK9 antibody
Mouse monoclonal NEK9 antibody
Product Categories/Family for anti-NEK9 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
107,168 Da
NCBI Official Full Name
serine/threonine-protein kinase Nek9
NCBI Official Synonym Full Names
NIMA-related kinase 9
NCBI Official Symbol
NEK9
NCBI Official Synonym Symbols
Nek8; NERCC; NERCC1
NCBI Protein Information
serine/threonine-protein kinase Nek9
UniProt Protein Name
Serine/threonine-protein kinase Nek9
UniProt Gene Name
NEK9
UniProt Synonym Gene Names
KIAA1995; NEK8; NERCC; NimA-related protein kinase 9; Nek8
UniProt Entry Name
NEK9_HUMAN
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Product Notes
The NEK9 nek9 (Catalog #AAA107091) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NEK9 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the NEK9 nek9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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