Mouse Nestin Monoclonal Antibody | anti-NES antibody

Anti-Nestin

Cat. Num. AAA14229

• Gene Names: NES; Nbla00170
• Applications: Western Blot, Immunofluorescence
• Purity: Protein G purified culture supernatant
• Synonyms: Nestin, Antibody; Anti-Nestin; anti-NES antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG₁
• Clone Number: 4D11
• Specificity: Specific for the ~220-240 kDa nestin doublet in Western blots of rat brain homogenate.
• Purity/Purification: Protein G purified culture supernatant
• Form/Format: 100ul in PBS + 50% Glycerol + 5 mM NaN₃. Adequate amount of material to conduct 10 mini Western blots.

• Applicable Applications for anti-NES antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:2,000; IF: 1:500
• Quality Control Tests: Western blots performed on each lot.
• Species Tested: Human, Mouse, Rat
• Immunogen: Recombinant human Nestin expressed in and purifeid from E. coli
• Preparation and Storage: Store at -20°C; stable for at least one year.

IS (Immunostaining)

(Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (AAA14229, red, 1:500) and anti-MAP2 antibody (, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.)

WB (Western Blot)

Related Product Information for anti-NES antibody

Target Description: Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells (Lendahl et al., 1990). Nestin was originally identified as a result of the production of a series of monoclonal antibodies directed against epitopes expressed on formalin fixed embryo day 15 rat spinal cord (Hockfield et al., 1985)). One of these antibodies, called Rat 401, stained fibrous profiles in the developing nervous system, but not in the mature nervous system. By screening bacteriophage expression libraries with Rat 401, Lendahl et al. were able to isolate a cDNA encoding the protein to which Rat 401 antibody bound. The protein proved to be an unusual member of the intermediate filament family, containing an alpha-helical region homologous to that found in keratin and neurofilament subunits.

References

• Lendahl U, Zimmerman LB and McKay RD. CNS stem cells express a new class of intermediate filament protein. Cell 60:585-95 (1990).
• Hockfield S and McKay RD. Identification of major cell classes in the developing mammalian nervous system. J. Neurosci. 5:3310-3328 (1985).

NCBI and Uniprot Product Information

• NCBI GI #: 38176300
• NCBI GeneID: 10763
• NCBI Accession #: NP_006608.1
• NCBI GenBank Nucleotide #: NM_006617.1
• UniProt Accession #: P48681; O00552; Q3LIF5; Q5SYZ6
• Molecular Weight: 220-240 kDa
• NCBI Official Full Name: nestin
• NCBI Official Synonym Full Names: nestin
• NCBI Official Symbol: NES
• NCBI Official Synonym Symbols: Nbla00170
• NCBI Protein Information: nestin
• UniProt Protein Name: Nestin
• UniProt Gene Name: NES
• UniProt Entry Name: NEST_HUMAN