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IHC (Immunohistochemistry)
(NES/Nestin Antibody-Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit anti-Mouse, Human NES/Nestin Polyclonal Antibody | anti-NES antibody
NES/Nestin Rabbit anti-Human Polyclonal Antibody
Reactivity
Mouse, Human
Applications
Western Blot, Immunohistochemistry
Purity
Immunoaffinity chromatography
Synonyms
NES/Nestin, Antibody; NES/Nestin Rabbit anti-Human Polyclonal Antibody; PathPlus NES/Nestin Antibody; NES; Nestin; Nbla00170; anti-NES antibody
Host
Rabbit
Reactivity
Mouse, Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Nestin protein.
Purity/Purification
Immunoaffinity chromatography
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH7.3, 30% glycerol, and 0.01% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-NES antibody
WB (Western Blot), IHC (Immunohistochemistry)
Target
Human NES/Nestin
Immunogen
Recombinant full length protein of human Nestin
Conjugation
Unconjugated
Preparation and Storage
Aliquot and store at -20 degree C for 1 year. Avoid freeze/thaw cycles.
IHC (Immunohistochemistry)
(NES/Nestin Antibody-Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(NES/Nestin Antibody-Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemisry)
(NES/Nestin Antibody-Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemisry)
(NES/Nestin Antibody-Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohiostchemistry)
(NES/Nestin Antibody-HumanKidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohiostchemistry)
(NES/Nestin Antibody-HumanKidney: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(NES/Nestin Antibody)
WB (Western Blot)
(NES/Nestin Antibody)
Related Product Information for anti-NES antibody
Nestin antibody is an unconjugated rabbit polyclonal antibody to Nestin (NES) from human. It is reactive with human and mouse. Validated for IHC and WB.
Nestin is an intermediate filament protein involved in regulating filament assembly, cell remodeling, regeneration, and neural development during embryogenesis. It is a marker of cell migration and proliferation and of progenitor neural cells. Nestin is mostly expressed in cells during development and expression is lost in terminally differentiated cells. It is also expressed in regenerating tissues and as part of the stress response to injury. It stains positively in progenitor neuroepithelial stem cells in the central nervous system (CNS) and in precursor cells of the peripheral nervous system (PNS), and in a subset of adult neurons in the basal forebrain. Nestin also sees expression outside of the CNS and PNS, generally in cells that are pluripotent or undifferentiated, highly mobile, or responding to injury or disease. It may show positivity in endothelial cells, myoepithelial cells, renal glomeruli, cardiac myocytes, the retina, striated muscle, the pancreas, the liver, kidneys, skin, teeth, testes, and the adrenal gland. In cancer, it is expressed in various tumors of the central nervous system, including astrocytic gliomas, neurocytomas, neuroblastomas, ependymomas, medulloblastomas and Schwannomas.
Nestin is an intermediate filament protein involved in regulating filament assembly, cell remodeling, regeneration, and neural development during embryogenesis. It is a marker of cell migration and proliferation and of progenitor neural cells. Nestin is mostly expressed in cells during development and expression is lost in terminally differentiated cells. It is also expressed in regenerating tissues and as part of the stress response to injury. It stains positively in progenitor neuroepithelial stem cells in the central nervous system (CNS) and in precursor cells of the peripheral nervous system (PNS), and in a subset of adult neurons in the basal forebrain. Nestin also sees expression outside of the CNS and PNS, generally in cells that are pluripotent or undifferentiated, highly mobile, or responding to injury or disease. It may show positivity in endothelial cells, myoepithelial cells, renal glomeruli, cardiac myocytes, the retina, striated muscle, the pancreas, the liver, kidneys, skin, teeth, testes, and the adrenal gland. In cancer, it is expressed in various tumors of the central nervous system, including astrocytic gliomas, neurocytomas, neuroblastomas, ependymomas, medulloblastomas and Schwannomas.
References
Journal of Clinical Pathology 2005;58:222-223; PLoS ONE. 2010 Dec. 6(4): e18535, DOI:10.1371/journal.pone.0018535; J Interdiscipl Histopathol. 2016. 4, DOI: 10.5455/jihp.20160420021353; Histol Histopathol. 2005 Apr;20(2):665-71; Neuroscience and Behavioral Physiology. 2008 Feb. 38(2):165 -169, DOI: 10.1007/s11055-008-0025-z;
NCBI and Uniprot Product Information
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Product Notes
The NES nes (Catalog #AAA163331) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NES/Nestin Rabbit anti-Human Polyclonal Antibody reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's NES/Nestin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the NES nes for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NES/Nestin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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