Mouse anti-Human Nestin Monoclonal Antibody | anti-NES antibody

Nestin (Cancer Stem Cell Marker)

Cat. Num. AAA214883

• Gene Names: NES; Nbla00170
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Nestin, Antibody; Nestin (Cancer Stem Cell Marker); ESTM 46; FLJ21841; Intermediate filament protein; Nbla00170; nes; anti-NES antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: NES/2911
• Specificity: Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or -internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differentiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 1621

• Applicable Applications for anti-NES antibody: IHC (Immunohistochemistry)
• Positive Control: Human kidney, ovary, cervix, breast, colon, endometrium, cerebellum, lung, prostate, skin and human melanoma tissue. HeLa cell lysate. SH-SY5Y cells.
• Immunogen: Recombinant human NES protein fragment around aa 698-844 (exact sequence is proprietary)
• Cellular Localization: Cytoplasm and membrane.
• Hu-chromosome Location: 1q23.1
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified Nestin Mouse Monoclonal Antibody (NES/2911). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Nestin Mouse Monoclonal Antibody (NES/2911).)

IHC (Immunohistochemisry)

(Formalin-fixed, paraffin-embedded human Kidney stained with Nestin Mouse Monoclonal Antibody (NES/2911).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Kidney stained with Nestin Mouse Monoclonal Antibody (NES/2911).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Nestin Mouse Monoclonal Antibody (NES/2911).)

References

1. Yamahatsu, K., et al. (2012). International Journal of Oncology, 40, 1345-1357. 2. Zhang Y, et al. Nat Commun. 2018 Sep 6;9(1):3613

NCBI and Uniprot Product Information

• NCBI GI #: 146345464
• NCBI GeneID: 10763
• UniProt Accession #: P48681
• Molecular Weight: 190-200kDa
• NCBI Official Full Name: Nestin
• NCBI Official Synonym Full Names: nestin
• NCBI Official Symbol: NES
• NCBI Official Synonym Symbols: Nbla00170
• NCBI Protein Information: nestin
• UniProt Protein Name: Nestin
• UniProt Gene Name: NES
• UniProt Entry Name: NEST_HUMAN