WB (Western Blot)
(Detection of mouse NF-H by western blot. Samples: Whole cell lysate (50 ug) from CT26, NIH 3T3, ND7/23 (10 ug), TCMK-1, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NF-H recombinant monoclonal antibody (AAA213634 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
NF-H recombinant antibody
Rabbit anti-NF-H Recombinant Monoclonal Antibody [BLR190J]
Gene Names
Nefh; Nfh; NF-H; NF200; mKIAA0845
Reactivity
Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
NF-H, Recombinant Antibody; Rabbit anti-NF-H Recombinant Monoclonal Antibody [BLR190J]; Nfh; NF200; neurofilament triplet H protein; neurofilament heavy polypeptide; neurofilament 200kDa; 200 kDa neurofilament protein; NF-H; mKIAA0845; Neurofilament heavy polypeptide; neurofilament, heavy polypeptide; NF-H recombinant antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Clone Number
BLR190J
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
50ug/ml (varies by lot)
Applicable Applications for NF-H recombinant antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1040 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse NF-H by western blot. Samples: Whole cell lysate (50 ug) from CT26, NIH 3T3, ND7/23 (10 ug), TCMK-1, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NF-H recombinant monoclonal antibody (AAA213634 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of mouse NF-H by western blot. Samples: Whole cell lysate (50 ug) from CT26, NIH 3T3, ND7/23 (10 ug), TCMK-1, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NF-H recombinant monoclonal antibody (AAA213634 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of mouse NF-H by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from ND7/23 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-NF-H recombinant monoclonal antibody (AAA213634 lot 1) used for IP at 20 ul/mg lysate. NF-H was also immunoprecipitated by a second antibody against a different epitope of NF-H (BL-2008A-1E7). For blotting immunoprecipitated NF-H, AAA213634 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of mouse NF-H by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from ND7/23 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-NF-H recombinant monoclonal antibody (AAA213634 lot 1) used for IP at 20 ul/mg lysate. NF-H was also immunoprecipitated by a second antibody against a different epitope of NF-H (BL-2008A-1E7). For blotting immunoprecipitated NF-H, AAA213634 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of mouse NF-H in mouse brain by IHC. Antibody: Rabbit anti-NF-H recombinant monoclonal antibody (AAA213634 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
IHC (Immunohistochemistry)
(Detection of mouse NF-H in mouse brain by IHC. Antibody: Rabbit anti-NF-H recombinant monoclonal antibody (AAA213634 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
Related Product Information for NF-H recombinant antibody
Neurofilaments are type IV intermediate filament heteropolymers composed of light (L), medium (M), and heavy (H) chains. Neurofilaments are a component of the axon cytoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. NF-H is commonly used as a biomarker of neuronal damage [taken from NCBI Entrez Gene (Gene ID: 4744)].
Product Categories/Family for NF-H recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
116,994 Da
NCBI Official Full Name
neurofilament heavy polypeptide
NCBI Official Synonym Full Names
neurofilament, heavy polypeptide
NCBI Official Symbol
Nefh
NCBI Official Synonym Symbols
Nfh; NF-H; NF200; mKIAA0845
NCBI Protein Information
neurofilament heavy polypeptide; 200 kDa neurofilament protein; neurofilament 200kDa; neurofilament triplet H protein
UniProt Protein Name
Neurofilament heavy polypeptide
UniProt Gene Name
Nefh
UniProt Synonym Gene Names
Kiaa0845; Nfh; NF-H
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Product Notes
The NF-H nefh (Catalog #AAA213634) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NF-H Recombinant Monoclonal Antibody [BLR190J] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NF-H can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NF-H nefh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NF-H, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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