NF kappa B p100 Monoclonal Antibody | anti-NFkappaB antibody

NF kappa B p100 Rabbit mAb

Cat. Num. AAA314422

• Gene Names: NFKB2; p52; p105; H2TF1; LYT10; LYT-10; NF-kB2
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot
• Purity: Affinity Purified
• Synonyms: NF kappa B p100, Antibody; NF kappa B p100 Rabbit mAb; p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2; p49/p100; anti-NFkappaB antibody

• Host: Recombinant Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: S04-4D6
• Purity/Purification: Affinity Purified
• Form/Format: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: 0.3mg/ml (varies by lot)

• Applicable Applications for anti-NFkappaB antibody: IF (Immunofluorescence), WB (Western Blot)
• Immunogen: Recombinant protein of human NFkB p100
• Conjugation: Unconjugated
• Modification: Unmodification
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence of NFkB p100(green) in Hela cells using NFkB p100 Rabbit mAb at dilution 1/200, and DAPI(blue))

WB (Western Blot)

(Western blot detection of NFkB p100/NFKB2 in C6,3T3,Hela cell lysates using NFkB p100/NFKB2 Rabbit mAb(1:1000 diluted).Predicted band size:97kDa.Observed band size:100kDa.)

Related Product Information for anti-NFkappaB antibody

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.

Product Categories/Family for anti-NFkappaB antibody

Primary Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 117320531
• NCBI GeneID: 4791
• NCBI Accession #: NP_001070962.1
• NCBI GenBank Nucleotide #: NM_001077494.2
• UniProt Accession #: Q00653; Q04860; Q9BU75; Q9H471; Q9H472; A8K9D9; D3DR83; AAB23437
• Molecular Weight: 96,749 Da
• NCBI Official Full Name: nuclear factor NF-kappa-B p100 subunit isoform a
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• NCBI Official Symbol: NFKB2
• NCBI Official Synonym Symbols: p52; p105; H2TF1; LYT10; LYT-10; NF-kB2
• NCBI Protein Information: nuclear factor NF-kappa-B p100 subunit; oncogene Lyt-10; DNA-binding factor KBF2; lymphocyte translocation chromosome 10 protein; nuclear factor of kappa light chain gene enhancer in B-cells 2
• UniProt Protein Name: Nuclear factor NF-kappa-B p100 subunit
• UniProt Gene Name: NFKB2
• UniProt Synonym Gene Names: LYT10; Lyt10
• UniProt Entry Name: NFKB2_HUMAN