Mouse NF200 Monoclonal Antibody | anti-NEFH antibody
Anti-NF200 antibody (monoclonal)
Gene Names
NEFH; NFH
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Synonyms
NF200, Antibody; Anti-NF200 antibody (monoclonal); Neurofilament heavy polypeptide; neurofilament, heavy polypeptide; NFH; KIAA0845; 200 kDa neurofilament protein; Neurofilament triplet H protein; anti-NEFH antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
N52
Specificity
No cross reactivity with other proteins.
Form/Format
Lyophilized
Sequence Length
1026
Applicable Applications for anti-NEFH antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin
Application Notes
Western Blot
Concentration : 0.5 ug/mL
Tested Species : Human,Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
Concentration: 1-2 ug/mL
Tested Species: Human, Mouse, Rat
Antigen Retrieval: By Heat
Immunohistochemistry (Frozen Section)
Concentrarion: 1-2 ug/mL
Tested Species: Human, Mouse, Rat
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Concentration : 0.5 ug/mL
Tested Species : Human,Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
Concentration: 1-2 ug/mL
Tested Species: Human, Mouse, Rat
Antigen Retrieval: By Heat
Immunohistochemistry (Frozen Section)
Concentrarion: 1-2 ug/mL
Tested Species: Human, Mouse, Rat
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen
C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Preparation and Storage
At -20°C for one year. After reconstitution at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Related Product Information for anti-NEFH antibody
Background: Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD(NFL), 125 kD(NFM), and 200 kD(NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein(NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions(exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease(CMT).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Neurofilament heavy polypeptide
NCBI Official Synonym Full Names
neurofilament, heavy polypeptide
NCBI Official Symbol
NEFH
NCBI Official Synonym Symbols
NFH
NCBI Protein Information
neurofilament heavy polypeptide; NF-H; 200 kDa neurofilament protein; neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa
UniProt Protein Name
Neurofilament heavy polypeptide
UniProt Gene Name
NEFH
UniProt Synonym Gene Names
KIAA0845; NFH; NF-H
UniProt Entry Name
NFH_HUMAN
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Product Notes
The NEFH nefh (Catalog #AAA11507) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-NF200 antibody (monoclonal) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NF200 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin. Western Blot Concentration : 0.5 ug/mL Tested Species : Human,Mouse, Rat Immunohistochemistry (Paraffin-embedded Section) Concentration: 1-2 ug/mL Tested Species: Human, Mouse, Rat Antigen Retrieval: By Heat Immunohistochemistry (Frozen Section) Concentrarion: 1-2 ug/mL Tested Species: Human, Mouse, Rat Other applications have not been tested. Optimal dilutions should be determined by end users. . Researchers should empirically determine the suitability of the NEFH nefh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NF200, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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