Mouse anti-Human NFATc1 Monoclonal Antibody | anti-NFATc1 antibody

NFATc1 antibody

Cat. Num. AAA48044

• Gene Names: NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
• Purity: By protein-G affinity chromatography
• Synonyms: NFATc1, Antibody; NFATc1 antibody; nuclear factor of activated T-cells; cytosolic component 1 isoform A; NF-ATC; NFATc; NFAT2; anti-NFATc1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, k
• Clone Number: AT1C3
• Purity/Purification: By protein-G affinity chromatography
• Form/Format: Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 716

• Applicable Applications for anti-NFATc1 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
• Antigen Species: Human
• Immunogen: Recombinant human NFATc1 (428-716aa) purified from E. coli
• Clone Note: Anti-human NFATc1 mAb, clone AT1C3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NFATc1 protein.
• Preparation and Storage: Can be stored at 4 degree C.; For long term storage, aliquot and store at -20 degree C.; Avoid repeated freezing and thawing cycles.

IF (Immunofluorescence)

(ICC/IF analysis of NFATc1 in HaLe cells. The cell was stained with ATGA0133 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

WB (Western Blot)

(The cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NFATc1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: NFATc1 transfected 293T cell lysate)

Related Product Information for anti-NFATc1 antibody

Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATc1) is a component of the nuclear factor of activated T cells DNA-binding transcription complex which consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. This protein plays a main role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription and functions as a major molecular target for the immunosuppressive drugs such as cyclosporine A. NFATc1 is expressed in most human primary lymphocytes and mature human T- and B-cell neoplasms.

Product Categories/Family for anti-NFATc1 antibody

Transcription & Translation

References

Akimzhanov A, et al., (2008) Am J Pathol 172 (1):215-224; Asagiri M, et al., (2005) J Exp Med 202 (9):1261-1269; Rao A, et al., (1997) Annu Rev Immunol 15:707-747

NCBI and Uniprot Product Information

• NCBI GI #: 27502393
• NCBI GeneID: 4772
• NCBI Accession #: NP_765978
• NCBI GenBank Nucleotide #: NM_172390.2
• UniProt Accession #: O95644; Q12865; Q15793; Q2M1S3; B5B2M4; B5B2M5; B5B2M6; B5B2M7; B5B2M8; B5B2M9; B5B2N1
• Molecular Weight: 38,626 Da
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 1 isoform A
• NCBI Official Synonym Full Names: nuclear factor of activated T cells 1
• NCBI Official Symbol: NFATC1
• NCBI Official Synonym Symbols: NFAT2; NFATc; NF-ATC; NF-ATc1.2
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 1
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 1
• UniProt Gene Name: NFATC1
• UniProt Synonym Gene Names: NFAT2; NFATC; NF-ATc1; NFATc1; NF-ATc; NFATc