Application Data
(IF analysis of Hela with E12-138(Left) and DAPI (Right) diluted at 1:100.)
NFkappaB p65 Monoclonal Antibody
NFkappaB p65 Mouse Monoclonal Antibody
Reactivity
Human, Rat, Mouse
Synonyms
NFkappaB p65, Antibody; NFkappaB p65 Mouse Monoclonal Antibody; anti-NFkappaB p65 antibody
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Clone Number
5G6
Specificity
NFkappaB p65 Mouse monoclonal antibody detects endogenous p65 proteins.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Preparation and Storage
Store at-20 degree C for 1 year. Do not aliquot the antibody.
Application Data
(IF analysis of Hela with E12-138(Left) and DAPI (Right) diluted at 1:100.)
Application Data
(IF analysis of Hela with E12-138(Left) and DAPI (Right) diluted at 1:100.)
Application Data
(Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Mouse Spleen Tissue with E12-138 diluted at 1:2000.1Input: Hela Cell Lysate2IP product: IP dilute 1:200Western blot analysis: primary antibody : E12-138 1:2,000Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000)
Application Data
(Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Mouse Spleen Tissue with E12-138 diluted at 1:2000.1Input: Hela Cell Lysate2IP product: IP dilute 1:200Western blot analysis: primary antibody : E12-138 1:2,000Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000)
Application Data
Application Data
Related Product Information for anti-NFkappaB p65 antibody
NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.
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Product Notes
The NFkappaB p65 (Catalog #AAA267145) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The NFkappaB p65 Mouse Monoclonal Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. It is sometimes possible for the material contained within the vial of "NFkappaB p65, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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