Mouse anti-Human NM23-H1 Monoclonal Antibody | anti-NM23-H1 antibody

NM23-H1

Cat. Num. AAA48348

• Gene Names: NME1; NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
• Purity: By protein-A affinity chromatography
• Synonyms: NM23-H1, Antibody; NM23-H1; Non-metastatic cells 1; Nucleoside diphosphate kinase A; NDP kinase A; AWD; GAAD; NB; NBS; NDPK-A; NM23; Non-metastatic cells 1 AWD; drosophila; homolog of; NDPKA; NM23-M1; Granzyme A activated DNase; GZMA activated DNase; Metastasis inhibition factor nm23; NDK A; NDP Kinase A; NM23 long variant; included; Nonmetastatic protein 23; NME1-NME2 spliced read-through transcript; protein (NM23A) expressed in; Nonmetastatic cells 1; protein expressed in; NME1; homolog 1; NM23H1B; Tumor metastatic process-associated protein; anti-NM23-H1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b kappa
• Clone Number: AT5F4
• Purity/Purification: By protein-A affinity chromatography
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 152

• Applicable Applications for anti-NM23-H1 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
• Antigen Species: Human
• Immunogen: Recombinant human NME1 (1-152aa) purified from E. coli
• Conjugation: Unconjugated
• Note: This product was produced from tissue culture supernatant.
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

WB (Western Blot)

(The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NM23-H1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant human NME1 protein Lane 2.: Recombinant human NME2 protein Lane 3.: Recombinant human NME3 protein Lane 4.: Recombinant human NME4 protein)

ICC (Immunocytochemistry)

(ICC/IF analysis of NM23-H1 in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

Related Product Information for anti-NM23-H1 antibody

Non-metastatic cells 1 (NME1), also known as NM23-H1, originally identified as a candidate metastasis suppressor gene. NME1 is expressed in different tumor types where their levels have been alternatively associated with reduced or increased metastatic potential. Reductions in NME1 expression have been significantly associated with aggressive behavior in melanoma, breast, colon, and gastric carcinomas. On the contrary, high levels of NME1 gene expression are noted in the advanced stage of thyroid carcinomas.

Product Categories/Family for anti-NM23-H1 antibody

Epigenetics and Nuclear Signaling

References

Tee YT., et al. (2006) Taiwan J Obstet Gynecol. 45(2): 107-13.; Negroni A., et al. (2000) Cell Death Differ. 7(9): 843-50.

NCBI and Uniprot Product Information

• NCBI GI #: 4557797
• NCBI GeneID: 4830
• NCBI Accession #: NP_000260
• NCBI GenBank Nucleotide #: NP_000260.1
• UniProt Accession #: P15531
• NCBI Official Full Name: nucleoside diphosphate kinase A isoform b
• NCBI Official Synonym Full Names: NME/NM23 nucleoside diphosphate kinase 1
• NCBI Official Symbol: NME1
• NCBI Official Synonym Symbols: NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1
• NCBI Protein Information: nucleoside diphosphate kinase A
• UniProt Protein Name: Nucleoside diphosphate kinase A
• UniProt Gene Name: NME1
• UniProt Synonym Gene Names: NDPKA; NM23; NDK A; NDP kinase A; GAAD
• UniProt Entry Name: NDKA_HUMAN