FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NO66(652) with M25089 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (blue) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Mouse anti-Human NO66 Monoclonal Antibody | anti-NO66 antibody
NO66 (652) Mouse mAb
Gene Names
RIOX1; ROX; NO66; JMJD9; MAPJD; URLC2; hsNO66; C14orf169
Reactivity
Human
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay, Western Blot
Synonyms
NO66, Antibody; NO66 (652) Mouse mAb; NO66(652) Mouse mAb; 60S ribosomal protein L8 histidine hydroxylase; Bifunctional lysine-specific demethylase and histidyl-hydroxylase NO66; Histone lysine demethylase NO66; hsNO66; lysine-specific demethylase NO66; MAPJD; MYC-associated protein with JmjC domain; NO66; Nucleolar protein 66; Ribosomal oxygenase 1; Ribosomal oxygenase NO66; ROX; up-regulated in lung cancer 2; C14orf169; RIOX1; URLC2; anti-NO66 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Sequence Length
641
Applicable Applications for anti-NO66 antibody
IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), WB (Western Blot)
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic peptide(KLH coupled)corresponding to amino-terminal residues of human NO66
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NO66(652) with M25089 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (blue) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NO66(652) with M25089 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (blue) and an unlabelled control (cells without incubation with primary antibody, green). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
WB (Western Blot)
(NO66(652) was immunopre-cipitated from 1mg of Jurkat cells membrane fraction, blotted with M25089 of 10 ug. Western blot was performed from the immunopre-cipitate using M25089 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 71 KDaObserved band size : 71 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
WB (Western Blot)
(NO66(652) was immunopre-cipitated from 1mg of Jurkat cells membrane fraction, blotted with M25089 of 10 ug. Western blot was performed from the immunopre-cipitate using M25089 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 71 KDaObserved band size : 71 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
Related Product Information for anti-NO66 antibody
Oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. Specifically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and monomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216'. Acts as a regulator of osteoblast differentiation via its interaction with SP7, OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7, OSX-mediated promoter activation (By similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation.
Product Categories/Family for anti-NO66 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted MW: 71kDa
NCBI Official Full Name
ribosomal oxygenase 1
NCBI Official Synonym Full Names
ribosomal oxygenase 1
NCBI Official Symbol
RIOX1
NCBI Official Synonym Symbols
ROX; NO66; JMJD9; MAPJD; URLC2; hsNO66; C14orf169
NCBI Protein Information
ribosomal oxygenase 1
UniProt Protein Name
Ribosomal oxygenase 1
UniProt Gene Name
RIOX1
UniProt Synonym Gene Names
C14orf169; MAPJD; NO66; hsNO66; ROX
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Product Notes
The NO66 riox1 (Catalog #AAA62389) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NO66 (652) Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NO66 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the NO66 riox1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NO66, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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