FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with NQO1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Rabbit NQO1 Monoclonal Antibody | anti-NQO1 antibody
NQO1 Antibody
Gene Names
NQO1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
NQO1, Antibody; NQO1 Antibody; Azoreductase antibody; Cytochrome b 5 reductase antibody; DHQU antibody; DIA 4 antibody; DIA4 antibody; Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody; Diaphorase (NADH/NADPH) antibody; Diaphorase 4 antibody; Dioxin inducible 1 antibody; DT diaphorase antibody; DT-diaphorase antibody; DTD antibody; Menadione reductase antibody; NAD (P)H dehydrogenase [quinone] 1 antibody; NAD (P)H dehydrogenase quinone 1 antibody; NAD (P)H menadione oxidoreductase 1 dioxin inducible antibody; NAD (P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody; NAD (P)H:menadione oxidoreductase 1 antibody; NAD (P)H:Quinone acceptor oxidoreductase type 1 antibody; NAD (P)H:quinone oxidoreductase 1 antibody; NAD (P)H:quinone oxireductase antibody; NMOR 1 antibody; NMOR I antibody; NMOR1 antibody; NMORI antibody; NQO 1 antibody; NQO1 antibody; NQO1_HUMAN antibody; Phylloquinone reductase antibody; QR 1 antibody; QR1 antibody; Quinone reductase 1 antibody; anti-NQO1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JF440-1
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-NQO1 antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with NQO1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with NQO1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
WB (Western Blot)
(Western blot analysis of NQO1 on different lysates using anti-NQO1 antibody at 1/1, 000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: Mouse kidney Lane 3: Raji)
WB (Western Blot)
(Western blot analysis of NQO1 on different lysates using anti-NQO1 antibody at 1/1, 000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: Mouse kidney Lane 3: Raji)
Related Product Information for anti-NQO1 antibody
NAD (P)H:quinone oxidoreductase 1 (NQO1) and NRH:quinone oxidoreductase (NQO2) are flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones, using either NADH or NADPH as the electron donor. This protects cells against quinone-induced oxidative stress, cytotoxicity, and mutagenicity. Many tumors overexpress NQO1, which is an obligate two-electron reductase that deactivates toxins and activates bioreductive anticancer drugs. NQO1, a 274 amino acid protein, is ubiquitously expressed, but the expression level varies among tissues. NQO1 gene expression is coordinately induced in response to xenobiotics, antioxidants, heavy metals and radiation. The antioxidant response element (ARE) in the NQO1 gene promoter is essential for expression and coordinated induction of NQO1. ARE activation by tert-butylhydroquinone is dependent on PI3-kinase, which lies upstream of Nrf2. Nrf2, c-Jun, Nrf1, Jun-B and Jun-D bind to the ARE and regulate expression and induction of NQO1 gene. Maf-Maf homodimers and possibly Maf-Nrf2 heterodimers play a role in negative regulation of ARE-mediated transcription, but Maf-Nrf1 heterodimers fail to bind with the NQO1 gene ARE and do not repress NQO1 transcription.
Product Categories/Family for anti-NQO1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
274
NCBI Official Full Name
NAD(P)H dehydrogenase
NCBI Official Synonym Full Names
NAD(P)H dehydrogenase, quinone 1
NCBI Official Symbol
NQO1
NCBI Official Synonym Symbols
DTD; QR1; DHQU; DIA4; NMOR1; NMORI
NCBI Protein Information
NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase [quinone] 1; azoreductase; diaphorase-4; DT-diaphorase; dioxin-inducible 1; menadione reductase; quinone reductase 1; phylloquinone reductase; NAD(P)H:quinone oxireductase; NAD(P)H:quinone oxidoredu
UniProt Protein Name
NAD(P)H dehydrogenase [quinone] 1
UniProt Gene Name
NQO1
UniProt Synonym Gene Names
DIA4; NMOR1; DTD; QR1
UniProt Entry Name
NQO1_HUMAN
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Product Notes
The NQO1 nqo1 (Catalog #AAA312417) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NQO1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NQO1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NQO1 nqo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NQO1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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