IF (Immunofluorescence)
(ICC/IF analysis of NQO2 in A549 cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NQO2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human, Mouse NQO2 Monoclonal Antibody | anti-NQO2 antibody
NQO2 antibody
Gene Names
NQO2; QR2; DHQV; DIA6; NMOR2
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-G affinity chromatography
Synonyms
NQO2, Antibody; NQO2 antibody; Ribosyldihydronicotinamide dehydrogenase; DHQV; DIA6; QR2; Ribosyldihydronicotinamide dehydrogenase Ox2; EC 1.10.99.2; MGC94180; NAD (P)H dehydrogenase quinone 2; NAD (P)H menadione oxidoreductase 1 dioxin inducible 2; NAD (P)H menadione oxidoreductase 2 dioxin inducible; NMOR2; NQO 2; NRH dehydrogenase; NRH:quinone oxidoreductase 2; OTTHuMP00000015948; OTTHuMP00000015949; OTTHuMP00000015953; Ox 2; Quinone; Quinone reductase 2; Ribosyldihydronicotinamide dehydrogenase.; anti-NQO2 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT1E3
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
231
Applicable Applications for anti-NQO2 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human NQO2 (1-231aa) purified from E. coli
Clone Note
Anti-human NQO2 mAb, clone AT1E3, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NQO2 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of NQO2 in A549 cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NQO2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of NQO2 in A549 cell line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NQO2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NQO2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: K562 cell lysate Lane 3.: A549 cell lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NQO2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: K562 cell lysate Lane 3.: A549 cell lysate)
Related Product Information for anti-NQO2 antibody
Quinone oxidoreductase (NQO1 and NQO2) are cytosolic proteins that catalyze metabolic redaction of quinines and derivates.NQO2 is inhibited by flavones such as quercetin. Also benzo (a)pyrene is another known inhibitor of NQO2. Even though overlapping substrates specificities have been observed for NQO1 and NQO2, such as for CB1954 activation, significant differences exist in relative affinities for the various substrates.The detoxification role of NQO2 has not been found, and it has no known endogenous biological substrates. However, NQO1 plays an important role in the detoxification of various endogenous and exogenous quinones, including estrogen quinines. Also NQO2 has a melatonin-binding site, which may explain the anti-oxidant role of melatonin related with circadian rhythm.
Product Categories/Family for anti-NQO2 antibody
References
Wu K., et al,. (1997) Arch Biochem Biophys. 347 (2):221-8.; Aiswal AK., et al,. (1994) J Biol Chem. 269 (20):14502-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,919 Da
NCBI Official Full Name
NAD(P)H dehydrogenase, quinone 2
NCBI Official Synonym Full Names
N-ribosyldihydronicotinamide:quinone reductase 2
NCBI Official Symbol
NQO2
NCBI Official Synonym Symbols
QR2; DHQV; DIA6; NMOR2
NCBI Protein Information
ribosyldihydronicotinamide dehydrogenase [quinone]
UniProt Protein Name
Ribosyldihydronicotinamide dehydrogenase [quinone]
UniProt Gene Name
NQO2
UniProt Synonym Gene Names
NMOR2; QR2
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Product Notes
The NQO2 nqo2 (Catalog #AAA48081) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NQO2 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NQO2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the NQO2 nqo2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NQO2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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