IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NSMCE2 protein using NSMCE2 antibody)
Mouse NSMCE2 Monoclonal Antibody | anti-NSMCE2 antibody
NSMCE2 antibody
Gene Names
NSMCE2; NSE2; MMS21; ZMIZ7; C8orf36
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
NSMCE2 antibody was purified by affinity chromatography.
Synonyms
NSMCE2, Antibody; NSMCE2 antibody; Monoclonal NSMCE2; Anti-NSMCE2; Non-Smc Element 2 Mms21 Homolog; C8orf36; FLJ32440; MMS21; NSE2; NSMCE2; NSMCE 2; NSMCE-2; anti-NSMCE2 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4H9
Purity/Purification
NSMCE2 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
247
Applicable Applications for anti-NSMCE2 antibody
WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Biological Significance
NSMCE2 is the E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. NSMCE2 acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.
Cross-Reactivity
Human
Immunogen
NSMCE2 antibody was raised in mouse using a full length recombinant protein of human NSMCE2 (NP_775956) produced in E Coli as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NSMCE2 protein using NSMCE2 antibody)
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NSMCE2 protein using NSMCE2 antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NSMCE2 protein (Right) or empty vector (Left) detected with NSMCE2 antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NSMCE2 protein (Right) or empty vector (Left) detected with NSMCE2 antibody)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant NSMCE2 protein (red) or empty vector (blue) stained using NSMCE2 antibody)
FCM/FACS (Flow Cytometry)
(Flow Cytometric analysis of HEK293T cells transfected with either recombinant NSMCE2 protein (red) or empty vector (blue) stained using NSMCE2 antibody)
Related Product Information for anti-NSMCE2 antibody
Mouse monoclonal NSMCE2 antibody
Product Categories/Family for anti-NSMCE2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,932 Da
NCBI Official Full Name
E3 SUMO-protein ligase NSE2
NCBI Official Synonym Full Names
non-SMC element 2, MMS21 homolog (S. cerevisiae)
NCBI Official Symbol
NSMCE2
NCBI Official Synonym Symbols
NSE2; MMS21; ZMIZ7; C8orf36
NCBI Protein Information
E3 SUMO-protein ligase NSE2
UniProt Protein Name
E3 SUMO-protein ligase NSE2
UniProt Gene Name
NSMCE2
UniProt Synonym Gene Names
C8orf36; MMS21; hMMS21; Non-SMC element 2 homolog
UniProt Entry Name
NSE2_HUMAN
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Product Notes
The NSMCE2 nsmce2 (Catalog #AAA106860) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NSMCE2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the NSMCE2 nsmce2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NSMCE2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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