Mouse Nucleolar Antigen (Marker For Human Cells) Monoclonal Antibody

Human Nucleolar Antigen (Marker For Human Cells)

Cat. Num. AAA215335

• Reactivity: Human; Does not react with Mouse, Rat or Cow.
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified Ab conjugated to CF488
• Synonyms: Nucleolar Antigen (Marker For Human Cells), Antibody; Human Nucleolar Antigen (Marker For Human Cells); Not Known; anti-Nucleolar Antigen (Marker For Human Cells) antibody

• Host: Mouse
• Reactivity: Human; Does not react with Mouse, Rat or Cow.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: NM95
• Specificity: This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
• Purity/Purification: Purified Ab conjugated to CF488
• Form/Format: Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide. Contact us if you require this Ab in a different format.

• Applicable Applications for anti-Nucleolar Antigen (Marker For Human Cells) antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Positive Control: Any human cells. Tissues.
• Immunogen: Nuclei of myeloid leukemia biopsy cells
• Cellular Localization: Nucleoli
• Preparation and Storage: Antibody with azide: store at 4 to 8 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of human Nucleolar Ag on 293T cells. Black: cells alone; Grey: Isotype Control; Green: CF488-labeled human Nucleolar Monoclonal Antibody (NM95).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CF488-labeled human Nucleolar Monoclonal Antibody (NM95).)

References

Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137