WB (Western Blot)
(Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediate filament protein VM4341 (lane 1), anti-Nucleoporin p62nM4361 (lane 2), anti-Hsp60 mitochondrial protein HM4381 (lane 3), and anti-Calnexin endoplasmic reticulum protein CM4371 (lane 4).)
Mouse Nucleoporin p62 Monoclonal Antibody | anti-NUP62 antibody
Nucleoporin p62 (N-terminal region) Antibody
Gene Names
NUP62; p62; IBSN; SNDI
Reactivity
Human, Rat, Mouse, Chicken
Applications
Immunocytochemistry, ELISA, Western Blot
Synonyms
Nucleoporin p62, Antibody; Nucleoporin p62 (N-terminal region) Antibody; Nucleoporin p62 (N-terminal region); Nucleoporin; anti-NUP62 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse, Chicken
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
M436
Specificity
This antibody detects a 62kDa* protein corresponding to the apparent molecular mass of nucleoporin p62 on SDS-PAGE immunoblots of human HeLa and rat A7r5 cells. In immunocytochemistry, anti-Nucleoporin p62 specifically stains nuclei and nuclear envelope in paraformaldehyde fixed and NP-40 permeabilized cells.
Form/Format
Mouse monoclonal purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-NUP62 antibody
ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Clone M436 was generated from a recombinant protein containing amino acid residues in the N-terminal region of human nucleoporin p62. This sequence has high homology to similar regions in rat, mouse, and chicken nucleoporin p62.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
WB (Western Blot)
(Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediate filament protein VM4341 (lane 1), anti-Nucleoporin p62nM4361 (lane 2), anti-Hsp60 mitochondrial protein HM4381 (lane 3), and anti-Calnexin endoplasmic reticulum protein CM4371 (lane 4).)
WB (Western Blot)
(Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediate filament protein VM4341 (lane 1), anti-Nucleoporin p62nM4361 (lane 2), anti-Hsp60 mitochondrial protein HM4381 (lane 3), and anti-Calnexin endoplasmic reticulum protein CM4371 (lane 4).)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Nucleoporin p62 in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The fixed cells were labeled with mouse monoclonal anti-Nucleoporin p62 (N-terminal region) and the antibody was detected using Goat anti-Mouse secondary antibodies conjugated to DyLight 488.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Nucleoporin p62 in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The fixed cells were labeled with mouse monoclonal anti-Nucleoporin p62 (N-terminal region) and the antibody was detected using Goat anti-Mouse secondary antibodies conjugated to DyLight 488.)
Related Product Information for anti-NUP62 antibody
Active transport of proteins and RNA into and out of the nucleus occurs via the nuclear pore complex (NPC). The NPC is formed by a multiprotein complex that includes nucleoporin proteins. Specific nuclear localization sequences found in proteins target proteins for active transport into the nucleus through the NPC. Nucleoporin p62 is the best characterized member of the family of nucleoporins found in the NPC. A tightly associated complex is formed by p62 and two other nucleoporins, p54 and p58. p54 binds to a carboxy-terminal coiled-coil domain of p62 and p58 binds to a dimer of p54. The amino-terminal domain of p62 contains a series of XFXFX repeats and is joined to the coiled-coil domain by a threonine-rich linker segment. The major role of p62 is maintenance of the structural integrity of NPCs.
References
Newmeyer, D.D. (1993) Curr Opin Cell Biol. 5(3):395.
Carmo-Fonseca M. et al. (1991) Eur J Cell Biol. 55(1):17-30.
Carmo-Fonseca M. et al. (1991) Eur J Cell Biol. 55(1):17-30.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
522
NCBI Official Full Name
Nuclear pore glycoprotein p62
NCBI Official Synonym Full Names
nucleoporin 62kDa
NCBI Official Symbol
NUP62
NCBI Official Synonym Symbols
p62; IBSN; SNDI
NCBI Protein Information
nuclear pore glycoprotein p62; nucleoporin 62kD; nucleoporin Nup62; 62 kDa nucleoporin
UniProt Protein Name
Nuclear pore glycoprotein p62
UniProt Gene Name
NUP62
UniProt Entry Name
NUP62_HUMAN
Similar Products
Product Notes
The NUP62 nup62 (Catalog #AAA71674) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Nucleoporin p62 (N-terminal region) Antibody reacts with Human, Rat, Mouse, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleoporin p62 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NUP62 nup62 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleoporin p62, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.