Mouse anti-Human Occludin Monoclonal Antibody | anti-OCLN antibody

Occludin (Marker of Early Blood Brain Barrier Damage)

Cat. Num. AAA214869

• Gene Names: OCLN; BLCPMG; PTORCH1; PPP1R115
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Occludin, Antibody; Occludin (Marker of Early Blood Brain Barrier Damage); BLCPMG; Occludin; OCLN; Phosphatase 1 regulatory subunit 115; PTORCH1; Tight junction protein occludin; anti-OCLN antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: OCLN/2181
• Specificity: Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue; in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Occludin is a tight junction protein that is a key structural component of the blood-brain barrier (BBB). Degradation of Occludin is frequently seen in ischemic stroke and contributes to BBB disruption.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 271

• Applicable Applications for anti-OCLN antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Positive Control: HepG2 cells. Kidney.
• Immunogen: Recombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)
• Cellular Localization: Cell Surface and Cytoplasmic
• Hu-chromosome Location: 5q13.2
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS_PAGE

(SDS-PAGE Analysis Purified Occludin Mouse Monoclonal Antibody (OCLN/2181). Confirmation of Purity and Integrity of Antibody.)

WB (Western Blot)

(Western Blot Analysis of MCF-7 cell lysate with Occludin Mouse Monoclonal Antibody (OCLN/2181).)

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human thyroid carcinoma stained with Occludin Mouse Monoclonal Antibody (OCLN/2181).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Occludin Mouse Monoclonal Antibody (OCLN/2181).)

References

Pan, R. et al. Blood Occludin Level as a Potential Biomarker for Early Blood Brain Barrier Damage Following Ischemic Stroke. Sci. Rep. 7, 40331; (2017)

NCBI and Uniprot Product Information

• NCBI GI #: 327478416
• NCBI GeneID: 100506658
• NCBI Accession #: NP_001192184.1
• NCBI GenBank Nucleotide #: NP_001192184.1
• UniProt Accession #: Q16625
• Molecular Weight: 60-82kDa
• NCBI Official Full Name: occludin isoform b
• NCBI Official Synonym Full Names: occludin
• NCBI Official Symbol: OCLN
• NCBI Official Synonym Symbols: BLCPMG; PTORCH1; PPP1R115
• NCBI Protein Information: occludin
• UniProt Protein Name: Occludin
• UniProt Gene Name: OCLN
• UniProt Entry Name: OCLN_HUMAN