ICC (Immunocytochemistry)
(Immunocytochemical labeling of Osteopontin in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with mouse monoclonal anti-Osteopontin (M574). The antibody was detected using goat anti-mouse DyLight 594.)
Mouse Osteopontin Monoclonal Antibody | anti-SPP1 antibody
Osteopontin (N-terminal region) Antibody
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human, Rat, Mouse
Applications
Immunocytochemistry, ELISA, Western Blot
Synonyms
Osteopontin, Antibody; Osteopontin (N-terminal region) Antibody; Osteopontin (N-terminal region); Osteopontin; anti-SPP1 antibody
Host
Mouse
Reactivity
Human, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M574
Specificity
Clone M574 was purified using Protein G chromatography. The antibody detects a 60kDa* protein corresponding to the molecular mass of Osteopontin on SDS-PAGE immunoblots of human MDA-MB-231 cells and human full length recombinant Osteopontin protein.
Form/Format
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-SPP1 antibody
ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Clone M574 was generated from a recombinant protein containing amino acid residues in the N-terminal region of human osteopontin. This sequence has high homology with rat and mouse osteopontin.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Osteopontin in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with mouse monoclonal anti-Osteopontin (M574). The antibody was detected using goat anti-mouse DyLight 594.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Osteopontin in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with mouse monoclonal anti-Osteopontin (M574). The antibody was detected using goat anti-mouse DyLight 594.)
WB (Western Blot)
(Western blot analysis of human recombinant osteopontin protein (lane 1 & 2) or human MDA-MB-231 cells (lane 3 & 4). The blots were probed with mouse monoclonal anti-Osteopontin (OM5741) at a dilution of 1:1000 (lane 1), 1:4000 (lane 2), 1:250 (lane 3), and 1:1000 (lane 4).)
WB (Western Blot)
(Western blot analysis of human recombinant osteopontin protein (lane 1 & 2) or human MDA-MB-231 cells (lane 3 & 4). The blots were probed with mouse monoclonal anti-Osteopontin (OM5741) at a dilution of 1:1000 (lane 1), 1:4000 (lane 2), 1:250 (lane 3), and 1:1000 (lane 4).)
Related Product Information for anti-SPP1 antibody
Osteopontin (OPN) is a 34kDa sialic acid rich member of small integrin-binding ligand N-linked glycoproteins. It is expressed in many different tissues and is post-translationally modified at multiple sites with both glycosylation and phosphorylation. The mature post-translationally modified protein is 60kDa. OPN is involved with cell survival, proliferation, invasion, and stem like behavior. OPN can interact with CD44, bind hydroxyapatite, and activates many integrins. These interactions are important for OPN function in cell matrix formation. A higher presence of OPN has been found in a variety of cancers, leading to increased tumor growth and metastasis. In addition, OPN is involved in type I immunity through its function as a cytokine where it can enhance production of interferon-gamma and interleukin-12 and reduce production of interleukin-10.
References
Shevde, L. et al. (2014) Matrix Biology 37:131
Sodek, J. et al. (2000) Crit Rev Oral Biol Med. 11(3):279
Sodek, J. et al. (2000) Crit Rev Oral Biol Med. 11(3):279
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,423 Da
NCBI Official Full Name
osteopontin isoform OPN-b
NCBI Official Synonym Full Names
secreted phosphoprotein 1
NCBI Official Symbol
SPP1
NCBI Official Synonym Symbols
OPN; BNSP; BSPI; ETA-1
NCBI Protein Information
osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I,
UniProt Protein Name
Osteopontin
UniProt Gene Name
SPP1
UniProt Synonym Gene Names
BNSP; OPN; SPP-1
UniProt Entry Name
OSTP_HUMAN
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Product Notes
The SPP1 spp1 (Catalog #AAA71677) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Osteopontin (N-terminal region) Antibody reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Osteopontin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SPP1 spp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Osteopontin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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