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product-image-AAA178814_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry of Osteopontin in paraffin-embedded Human colon cancer tissue using Osteopontin Rabbit mAb at dilution 1:50)

Rabbit Osteopontin Monoclonal Antibody | anti-SPP1 antibody

Recombinant Osteopontin Monoclonal Antibody

Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human, Rat, Hamster
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
Osteopontin, Antibody; Recombinant Osteopontin Monoclonal Antibody; BNSP; BSPI; ETA-1; OPN; Osteopontin; SPP1; anti-SPP1 antibody
Ordering
Host
Rabbit
Reactivity
Human, Rat, Hamster
Clonality
Monoclonal
Isotype
IgG
Clone Number
R08-1C5
Purity/Purification
Affinity Purified
Form/Format
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Concentration
300ug/mL (varies by lot)
Applicable Applications for anti-SPP1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide of human Osteopontin
Research Area
Cancer, Cardiovascular, Signal Transduction, Stem Cells
Preparation and Storage
Storage: -20 degree C/One year. Avoid freeze/thaw cycles.

IHC (Immunohiostchemistry)

(Immunohistochemistry of Osteopontin in paraffin-embedded Human colon cancer tissue using Osteopontin Rabbit mAb at dilution 1:50)

product-image-AAA178814_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry of Osteopontin in paraffin-embedded Human colon cancer tissue using Osteopontin Rabbit mAb at dilution 1:50)

WB (Western Blot)

(Western blot detection of Osteopontin in Hela,CHO-K1,C6,Jurkat cell lysates using Osteopontin Rabbit mAb(1:1000 diluted).Predicted band size:35kDa.Observed band size:35kDa.)

product-image-AAA178814_WB15.jpg WB (Western Blot) (Western blot detection of Osteopontin in Hela,CHO-K1,C6,Jurkat cell lysates using Osteopontin Rabbit mAb(1:1000 diluted).Predicted band size:35kDa.Observed band size:35kDa.)
Related Product Information for anti-SPP1 antibody
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-SPP1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,423 Da
NCBI Official Full Name
osteopontin isoform OPN-b
NCBI Official Synonym Full Names
secreted phosphoprotein 1
NCBI Official Symbol
SPP1
NCBI Official Synonym Symbols
OPN; BNSP; BSPI; ETA-1
NCBI Protein Information
osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I,
UniProt Protein Name
Osteopontin
UniProt Gene Name
SPP1
UniProt Synonym Gene Names
BNSP; OPN; SPP-1
UniProt Entry Name
OSTP_HUMAN

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Product Notes

The SPP1 spp1 (Catalog #AAA178814) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Osteopontin Monoclonal Antibody reacts with Human, Rat, Hamster and may cross-react with other species as described in the data sheet. AAA Biotech's Osteopontin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SPP1 spp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Osteopontin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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