Mouse p53 Tumor Suppressor Protein Monoclonal Antibody | anti-TP53 antibody

p53 Tumor Suppressor Protein Mouse Monoclonal Antibody

Cat. Num. AAA62574

• Gene Names: TP53; P53; BCC7; LFS1; TRP53
• Reactivity: Human, Monkey, Cow.; Others not known
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: p53 Tumor Suppressor Protein, Antibody; p53 Tumor Suppressor Protein Mouse Monoclonal Antibody; Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53; anti-TP53 antibody

• Host: Mouse
• Reactivity: Human, Monkey, Cow.; Others not known
• Clonality: Monoclonal
• Isotype: Mouse/ IgG2a+ Mouse/ IgG2b/ kappa
• Clone Number: BP53-12 + DO-7
• Specificity: Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. Its epitope maps within the N-terminus (aa 20-25) of p53 oncoprotein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
• Sequence Length: 393

• Applicable Applications for anti-TP53 antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Cellular Localization: Predominantly nuclear
• Immunogen: Recombinant human wild-type p53 protein (BP53-12) & Recombinant human wild type p53 protein expressed in E Coli (DO-7)
• Hu-Chromosome Location: 17p13.1
• Positive Control: MDA-MB-231 Cells. Breast or Colon carcinoma
• Preparation and Storage: Antibody with azide - store at 2-8°C.; Antibody is stable for 24 months. Non-hazardous.

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with p53 Monoclonal Antibody (BP53-12 + DO-7))

References

Bartek J et. al. Journal of Pathology, 1993, 169(1):27-34. Vojtesek B et al. 1992. J. Immunol. Methods. 151(1-2): 237-44. Stephen CW et al. 1995. J Mol. Biol. 248(1): 58-78.

NCBI and Uniprot Product Information

• NCBI GI #: 120407068
• NCBI GeneID: 7157
• NCBI Accession #: NP_000537.3
• NCBI GenBank Nucleotide #: NM_000546.5
• UniProt Accession #: P04637; Q15086; Q15087; Q15088; Q16535; Q16807; Q16808; Q16809; Q16810; Q16811; Q16848; Q2XN98
• Molecular Weight: 53kDa
• NCBI Official Full Name: cellular tumor antigen p53 isoform a
• NCBI Official Synonym Full Names: tumor protein p53
• NCBI Official Symbol: TP53
• NCBI Official Synonym Symbols: P53; BCC7; LFS1; TRP53
• NCBI Protein Information: cellular tumor antigen p53
• UniProt Protein Name: Cellular tumor antigen p53
• UniProt Gene Name: TP53
• UniProt Synonym Gene Names: P53
• UniProt Entry Name: P53_HUMAN