WB (Western Blot)
(Western blot analysis of extracts from serum-starved MCF7, treated with IGF-1 (100 ng/mL, 30 min), using Phospho-S6K1 (Thr389) rabbit monoclonal Antibody (#AAA258873) and other brands’ antibodies (company C, company P) at dilution of 1:1000, 1:10000 and 1:200000.)
p70 S6 Kinase 1 Recombinant Antibody | p70S6 recombinant antibody
Recombinant Phospho-p70 S6 Kinase 1 (Thr389) Antibody, Rabbit Monoclonal
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Protein A
Synonyms
p70 S6 Kinase 1, Recombinant Antibody; Recombinant Phospho-p70 S6 Kinase 1 (Thr389) Antibody, Rabbit Monoclonal; ribosomal protein S6 kinase, polypeptide 1; Anti-p70 S6KA Antibody; Anti-p70(S6K)-alpha Antibody; Anti-p70-alpha Antibody; Anti-p70-S6K Antibody; Anti-PS6K Antibody; Anti-RPS6KB1 Antibody; Anti-S6K Antibody; Anti-S6K-beta-1 Antibody; Anti-S6K1 Antibody; Anti-STK14A Antibody; p70S6 recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
#0001
Specificity
Human Phospho-p70 S6 Kinase 1 (Thr389)
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for p70S6 recombinant antibody
WB (Western Blot)
Immunogen
A synthetic phosphopeptide corresponding to residues around Thr389 of human p70 S6 kinase 1.
Preparation
The antibody is produced by immunizing rabbits with a synthetic phosphopeptide corresponding to residues around Thr389 of human p70 S6 kinase 1.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of extracts from serum-starved MCF7, treated with IGF-1 (100 ng/mL, 30 min), using Phospho-S6K1 (Thr389) rabbit monoclonal Antibody (#AAA258873) and other brands’ antibodies (company C, company P) at dilution of 1:1000, 1:10000 and 1:200000.)
WB (Western Blot)
(Western blot analysis of extracts from serum-starved MCF7, treated with IGF-1 (100 ng/mL, 30 min), using Phospho-S6K1 (Thr389) rabbit monoclonal Antibody (#AAA258873) and other brands’ antibodies (company C, company P) at dilution of 1:1000, 1:10000 and 1:200000.)
WB (Western Blot)
(Western blot analysis of extracts from serum-starved MCF7, untreated (line A); treated with IGF-1 (100 ng/mL, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-S6K1 (Thr389) rabbit monoclonal Antibody (#AAA258873) at 1:5000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts from serum-starved MCF7, untreated (line A); treated with IGF-1 (100 ng/mL, 30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-S6K1 (Thr389) rabbit monoclonal Antibody (#AAA258873) at 1:5000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts from serum-starved MCF7, untreated (line A); treated with IGF-1 (100 ng/mL, 30 min; +) (line B); treated with IGF-1 and ?-phosphatase (line C) using Phospho-S6K1 (Thr389) rabbit monoclonal Antibody (#AAA258873) at 1:5000 dilution(upper), or Anti-RPS6KB1 rabbit polyclonal antibody (lower) at 1:5000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts from serum-starved MCF7, untreated (line A); treated with IGF-1 (100 ng/mL, 30 min; +) (line B); treated with IGF-1 and ?-phosphatase (line C) using Phospho-S6K1 (Thr389) rabbit monoclonal Antibody (#AAA258873) at 1:5000 dilution(upper), or Anti-RPS6KB1 rabbit polyclonal antibody (lower) at 1:5000 dilution.)
Related Product Information for p70S6 recombinant antibody
PS6K, also known as RPS6KB1, is a serine/threonine-protein kinase. It belongs to the RSK (ribosomal s6 kinase) family. Members of this family function in signal transduction. PS6K is an isoform of p70 ribosomal S6 kinase (S6K). S6K can be activated by mitogenic stimuli such as growth factors, insulin and cytokines. It phosphorylates the ribosomal protein S6. PS6K also phosphorylates other proteins such as elF4B, eEF2K and SKAR. It is a crucial effector of mTOR(rapamycin) signaling. PS6K is dissociated from the EIF3 complex and activated upon mitogenic stimulation, phosphorylation by the mammalian target of mTOR complex 1 (mTORC1). Its active form then phosphorylates and activates several substrates in the preinitiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. PS6K also functions in cell proliferation, cell growth and cell cycle progression.
References
Panasyuk, et al. (2006) Nuclear export of PS6K II is regulated by protein kinase CK2 phosphorylation at Ser-17. J Biol Chem. 281(42):31188-201.Carnevalli L, et al. (2010) PS6K Plays a Critical Role in Early Adipocyte Differentiation. Dev Cell. 18 (5):763-74.
Grove JR, et al. (1991) Cloning and expression of two human p70 S6 kinase polypeptides differing only at their amino termini. Mol Cell Biol. 11(11):5541-50.
Grove JR, et al. (1991) Cloning and expression of two human p70 S6 kinase polypeptides differing only at their amino termini. Mol Cell Biol. 11(11):5541-50.
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Product Notes
The p70S6 (Catalog #AAA258873) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Phospho-p70 S6 Kinase 1 (Thr389) Antibody, Rabbit Monoclonal reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's p70 S6 Kinase 1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the p70S6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p70 S6 Kinase 1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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