Application Data
(Fig.2: SDS-PAGE analysis of purified LT-3D1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified LT-3D1 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Mouse anti-Human PAP Monoclonal Antibody | anti-ACPP antibody
anti-human Prostatic Acid Phosphatase (PAP) monoclonal antibody
Gene Names
ACPP; ACP3; 5'-NT; ACP-3
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
PAP, Antibody; anti-human Prostatic Acid Phosphatase (PAP) monoclonal antibody; anti-ACPP antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
LT-3D1
Specificity
anti-human PAP (PAcP, ACPP)
Purity/Purification
Protein G
Form/Format
Purified
Sequence Length
386
Applicable Applications for anti-ACPP antibody
ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry)
Buffer
phosphate buffered saline, pH 7.2
Immunogen
genetic immunisation with cDNA encoding human PAP
Selection
based on recognition of the complete native protein expressed on transfected
mammalian cells
Immunogen
cDNA
Preparation and Storage
Short term: 2 degree C - 8 degree C; long term: -20 degree C (avoid repeated freezing and thawing)
Application Data
(Fig.2: SDS-PAGE analysis of purified LT-3D1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified LT-3D1 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Application Data
(Fig.2: SDS-PAGE analysis of purified LT-3D1 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified LT-3D1 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using LT-3D1 Cat.# GM- 0907. BOSC23 cells were transiently transfected with an expression vector encoding either PAP (red curve) or an irrelevant protein (control transfectant: black curve). Binding of LT-3D1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with PAP transfected cells.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using LT-3D1 Cat.# GM- 0907. BOSC23 cells were transiently transfected with an expression vector encoding either PAP (red curve) or an irrelevant protein (control transfectant: black curve). Binding of LT-3D1 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with PAP transfected cells.)
Related Product Information for anti-ACPP antibody
Background: Human prostatic acid phosphatase (PAP) is a non-specific phosphomonoesterase, synthesised and secreted into seminal plasma under androgenic control. Human PAP is a 100 kDa glycoprotein containing two subunits of approximately 50 kDa each (1,2). It catalyses the dephosphorylation of organic monophosphate esters, demonstrating optimum activity at an acid pH. Produced by the prostatic epithelium, serum levels of PAP are very low in healthy individuals, but are often elevated in malignant and benign prostatic disease while it has been used as a marker of identification and therapy control of cancer of the prostate gland (3).
References
Ostrowski WS, Kuciel R (1994). Human prostatic acid phosphatase: selected properties and practical applications. Clin Chim Acta 226(2):121-9
Bilhartz DL, Tindall DJ, Oesterling JE (1991). Prostate-specific antigen and prostatic acid phosphatase: biomolecular and physiologic characteristics. Urology 38(2):95-102
Veeramani S, Yuan TC, Chen SJ, Lin FF, Petersen JE, Shaheduzzaman S, Srivastava S, MacDonald RG, Lin MF (2005). Cellular prostatic acid phosphatase: a protein tyrosine phosphatase involved in androgen-independent proliferation of prostate cancer. Endocr Relat Cancer 12(4):805-22
Bilhartz DL, Tindall DJ, Oesterling JE (1991). Prostate-specific antigen and prostatic acid phosphatase: biomolecular and physiologic characteristics. Urology 38(2):95-102
Veeramani S, Yuan TC, Chen SJ, Lin FF, Petersen JE, Shaheduzzaman S, Srivastava S, MacDonald RG, Lin MF (2005). Cellular prostatic acid phosphatase: a protein tyrosine phosphatase involved in androgen-independent proliferation of prostate cancer. Endocr Relat Cancer 12(4):805-22
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,443 Da
NCBI Official Full Name
prostatic acid phosphatase isoform PAP
NCBI Official Synonym Full Names
acid phosphatase, prostate
NCBI Official Symbol
ACPP
NCBI Official Synonym Symbols
ACP3; 5'-NT; ACP-3
NCBI Protein Information
prostatic acid phosphatase; TMPase; 5'-nucleotidase; ecto-5'-nucleotidase; thiamine monophosphatase; prostatic acid phosphotase
UniProt Gene Name
ACPP
UniProt Synonym Gene Names
PAP; 5'-NT; TMPase
UniProt Entry Name
PPAP_HUMAN
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Product Notes
The ACPP acpp (Catalog #AAA71488) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human Prostatic Acid Phosphatase (PAP) monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PAP can be used in a range of immunoassay formats including, but not limited to, ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the ACPP acpp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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