Mouse anti-Human, Mouse Parafibromin Monoclonal Antibody | anti-CDC73 antibody

Parafibromin

Cat. Num. AAA59299

• Gene Names: CDC73; HYX; FIHP; HPTJT; HRPT1; HRPT2; C1orf28
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry
• Synonyms: Parafibromin, Antibody; Parafibromin; anti-CDC73 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Form/Format: Parafibromin is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.
• Sequence Length: 531

• Applicable Applications for anti-CDC73 antibody: IHC (Immunohistochemistry)
• Localization: Nuclear
• Control: Parathyroid, Colon, Testis, Adrenal, Breast, Cervix, Lymphoblastic Lymphoma, Bladder TCC
• Precautions: 1. For professional users only. Ensure results are interpreted by a medical professional.; 2. This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent sodium azide build-up.; 3. Ensure proper handling procedures are used with reagent. Always wear proper laboratory equipment such as laboratory coat and gloves when handling reagents.; 4. Unused solution should be disposed of according to local and federal regulations.; 5. Do not ingest reagent. If reagent ingested, contact a poison control center immediately.; 6. For complete recommendations for handling biological specimens please refer to the CDC document, “Guidelines for Safe Work Practices in Human and Animal Laboratories” (4).
• Intended Use: This antibody is intended for use in Immunohistochemical applications on formalinfixed paraffin-embedded tissues (FFPE), frozen tissue sections and cell preparations. Interpretation of results should be performed by a qualified medical professional.
• Immunogen: Synthetic peptide corresponding to the N-residues of human parafibromin.
• Specimen Preparation: Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation to ensure best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended. Tissue should remain hydrated. Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.
• Staining Procedure: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides.; 2. Air dry for 2 hours at 58° C.; 3. Deparaffinize, dehydrate and rehydrate tissues.; 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.; 5. Any of three heating methods may be used:; a. TintoRetriever Pressure Cooker or Equivalent Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.; b. TintoRetriever PT Module or Water Bath Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.; c. Conventional Steamer Method Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.; 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.; 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.; 8. Wash slides with IHC wash buffer or DI water.; 9. Continue IHC staining protocol.
• Preparation and Storage: Store at 2-8°C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.

Application Data

IHC (Immunohistochemistry)

IHC (Immunohiostchemistry)

(IHC of Parafibromin on an FFPE Parathyroid CarcinomaTissue)

Dilution Info

Related Product Information for anti-CDC73 antibody

Cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S.cerevisiae), also known as CDC73 and parafibromin, is a protein which in humans is encoded by the CDC73 gene. Mutations in the CDC73 gene are associated with hyperparathyroidism-jaw tumor syndrome (HPT-JT) and parathyroid carcinomas.The proposed role of HRPT2 as a tumor suppressor was supported by mutation screening in parathyroid adenomas with cystic features, which identified three somatic inactivating mutations, all located in exon 1. None of these mutations were detected in normal controls, and all were predicted to cause deficient or impaired protein function. Sporadic parathyroid carcinomas frequently have HRPT2 mutations that are likely to be of pathogenetic importance. Certain patients with apparently sporadic parathyroid carcinoma carry germ-line mutations in HRPT2 and may have the HPT-JT syndrome or a phenotypic variant.

NCBI and Uniprot Product Information

• NCBI GI #: 40018640
• NCBI GeneID: 79577
• NCBI Accession #: NP_078805.3
• NCBI GenBank Nucleotide #: NM_024529.4
• Molecular Weight: 60,577 Da
• NCBI Official Full Name: parafibromin
• NCBI Official Synonym Full Names: cell division cycle 73
• NCBI Official Symbol: CDC73
• NCBI Official Synonym Symbols: HYX; FIHP; HPTJT; HRPT1; HRPT2; C1orf28
• NCBI Protein Information: parafibromin
• UniProt Protein Name: Parafibromin
• UniProt Gene Name: CDC73
• UniProt Synonym Gene Names: C1orf28; HRPT2
• UniProt Entry Name: CDC73_HUMAN