Mouse Parathyroid Hormone (PTH) Monoclonal Antibody | anti-PTH antibody

Parathyroid Hormone (PTH) (N-Terminal) Mouse Monoclonal Antibody

Cat. Num. AAA13828

• Gene Names: PTH; PTH1
• Reactivity: Human.; Predicted to react with Mouse, Rat, Rabbit, Cow, Dog, Pig, Deer and Orangutan
• Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry
• Synonyms: Parathyroid Hormone (PTH), Antibody; Parathyroid Hormone (PTH) (N-Terminal) Mouse Monoclonal Antibody; hPTH; Parathormone; Parathyrin; Parathyroid hormone 1 (PTH1); Parathyroid hormone (PTH); anti-PTH antibody

• Host: Mouse
• Reactivity: Human.; Predicted to react with Mouse, Rat, Rabbit, Cow, Dog, Pig, Deer and Orangutan
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: 3H9
• Specificity: Epitope of this MAb maps in between aa 1-34. PTH is a hormone produced by the parathyroid gland that regulates the concentration of calcium and phosphorus in extracellular fluid. This hormone elevates blood Ca2+ levels by dissolving the salts in bone and preventing their renal excretion. It is produced in the parathyroid gland as an 84 amino acid single chain polypeptide. It can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH); also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 115

• Applicable Applications for anti-PTH antibody: Flow Cytometry (FC), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Immunohistochemistry(Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Cellular Localization: Cytoplasmic and Secreted
• Immunogen: Synthetic peptide corresponding to amino acids 1 to 34 of mature PTH-polypeptide (exact sequence is propriatary)
• Hu-Chromosome Location: 11p15.3-p15.1
• Positive Control: Human parathyroid gland carcinoma
• Preparation and Storage: Antibody without azide - store at -20 to -80°C. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified PTH Mouse Monoclonal Antibody (3H9). Confirmation of Purity and Integrity of Antibody)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Parathyroid Gland stained with PTH Mouse Monoclonal Antibody (3H9).)

References

Watson, P.H. and Hanley, D.A. 1993. Parathyroid hormone: regulation of synthesis and secretion. Clin. Invest. Med. 16: 58-77.

NCBI and Uniprot Product Information

• NCBI GI #: 4506267
• NCBI GeneID: 5741
• NCBI Accession #: NP_000306.1
• NCBI GenBank Nucleotide #: NM_000315.3
• UniProt Accession #: P01270; Q4VB48; Q9UD38
• Molecular Weight: 9kDa
• NCBI Official Full Name: parathyroid hormone isoform 1 preproprotein
• NCBI Official Synonym Full Names: parathyroid hormone
• NCBI Official Symbol: PTH
• NCBI Official Synonym Symbols: PTH1
• NCBI Protein Information: parathyroid hormone
• UniProt Protein Name: Parathyroid hormone
• UniProt Gene Name: PTH
• UniProt Synonym Gene Names: PTH
• UniProt Entry Name: PTHY_HUMAN