Mouse anti-Human Parathyroid Hormone Receptor 1 Monoclonal Antibody
Mouse Anti-Parathyroid Hormone Receptor 1 [3D1.1]
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
Protein G purified
Synonyms
Parathyroid Hormone Receptor 1, Antibody; Mouse Anti-Parathyroid Hormone Receptor 1 [3D1.1]; MGC138426 antibody; MGC138452 antibody; Parathyroid hormone 1 receptor antibody; Parathyroidhormone/parathyroid hormone related peptide receptor antibody; Parathyroid hormone/parathyroid hormone relatedprotein receptor antibody; Parathyroid hormone/parathyroid hormone-related peptide receptor antibody; PTH receptorantibody; PTH/PTHr receptor antibody; PTH/PTHrP receptor antibody; PTH/PTHrP type I receptor antibody; PTH1receptor antibody; PTH1R antibody; PTH1R_HUMAN antibody; PTHR 1 antibody; PTHR antibody; PTHR1antibody; anti-Parathyroid Hormone Receptor 1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Ig
Clone Number
3979
Specificity
reacts with the human Type I hPTH/PTHrP and it does not react with the Type II receptor
Purity/Purification
Protein G purified
Form/Format
Purified antibody in PBS + 0.1% sodium azide
Concentration
0.1mg/ml (varies by lot)
Applicable Applications for anti-Parathyroid Hormone Receptor 1 antibody
IHC (Immunohistochemistry), WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA
Immunogen
23 amino acid peptide (corresponding to amino acids 146-169) of the hPTH/PTHrP receptor
Myeloma/fusion Partners
P3X63Ag8.653
Preparation and Storage
Store at 4°C for one month or in small aliquots at -20°C for longer periods. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Related Product Information for anti-Parathyroid Hormone Receptor 1 antibody
3D 1.1 reacts with the human Type I hPTH/PTHrP and it does not react with the Type II receptor. The antibody has been used in immunochemical studies of human ovarian tissues to demonstrate a novel role for PTH and/or PTHrP in ovarian function.
3D 1.1 recognises proteins with apparent molecular weights of 80,000 and 160,000 daltons in western blots of celllysates. Competitive binding assays show that the epitope recognized by 3D1.1 is localized to the C-terminal region of the immunogenic peptide. Furthermore, a corresponding peptide from the related PTH receptor (hPTH2) was shown to have minimal (0.1 %) cross reactivity. Finally, F ACS analysis has also been used to show that the antibody recognizes C-21 cells, but not parental BEK cells that express only the hPTH2 receptor.
Immunohistochemical staining of fixed, paraffin-embedded tissue shows specific hPTHlPTHrP receptor expression in chondrocytes, renal tubular and glomerular epithelium, afferent and efferent renal arterioles (but not glomerulr endothelium), and vascular smooth muscle and endothelium. This finding confirms that 3 D 1.1 positively stains classical PTH target tissues.
3D 1.1 recognises proteins with apparent molecular weights of 80,000 and 160,000 daltons in western blots of celllysates. Competitive binding assays show that the epitope recognized by 3D1.1 is localized to the C-terminal region of the immunogenic peptide. Furthermore, a corresponding peptide from the related PTH receptor (hPTH2) was shown to have minimal (0.1 %) cross reactivity. Finally, F ACS analysis has also been used to show that the antibody recognizes C-21 cells, but not parental BEK cells that express only the hPTH2 receptor.
Immunohistochemical staining of fixed, paraffin-embedded tissue shows specific hPTHlPTHrP receptor expression in chondrocytes, renal tubular and glomerular epithelium, afferent and efferent renal arterioles (but not glomerulr endothelium), and vascular smooth muscle and endothelium. This finding confirms that 3 D 1.1 positively stains classical PTH target tissues.
Similar Products
Product Notes
The Parathyroid Hormone Receptor 1 (Catalog #AAA59533) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Parathyroid Hormone Receptor 1 [3D1.1] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Parathyroid Hormone Receptor 1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the Parathyroid Hormone Receptor 1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Parathyroid Hormone Receptor 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.