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IP (Immunoprecipitation)
(PARK7 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate2 uL anti-PARK7 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PARK7 rabbit monoclonal antibody,at 1:1000 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa)
Rabbit anti-Human PARK7/DJ-1 Monoclonal Antibody | anti-PARK7 antibody
Recombinant Anti-PARK7/DJ-1 Antibody, Rabbit Monoclonal
Gene Names
PARK7; DJ1; DJ-1
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot
Purity
Protein A
Synonyms
PARK7/DJ-1, Antibody; Recombinant Anti-PARK7/DJ-1 Antibody, Rabbit Monoclonal; PARK7/DJ-1 Antibody, Rabbit MAb; parkinson protein 7; Anti-DJ-1 Antibody; Anti-DJ1 Antibody; Anti-HEL-S-67p Antibody; anti-PARK7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
007
Specificity
Human PARK7/DJ-1
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PARK7 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human PARK7/DJ-1 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human PARK7/DJ-1 (rh PARK7/DJ-1).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(PARK7 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate2 uL anti-PARK7 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PARK7 rabbit monoclonal antibody,at 1:1000 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa)
IP (Immunoprecipitation)
(PARK7 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg 293T Whole Cell Lysate2 uL anti-PARK7 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PARK7 rabbit monoclonal antibody,at 1:1000 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa)
WB (Western Blot)
(Anti-PARK7 rabbit monoclonal antibody at 1:1000 dilutionLane A: Hela Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:20 kDaObserved band size:24 kDa)
WB (Western Blot)
(Anti-PARK7 rabbit monoclonal antibody at 1:1000 dilutionLane A: Hela Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:20 kDaObserved band size:24 kDa)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human PARK7 expression on HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human PARK7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Human PARK7 expression on HeLa cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human PARK7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
IF (Immunofluorescence)
(Immunofluorescence staining of Human PARK7 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PARK7 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of Human PARK7 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PARK7 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
Related Product Information for anti-PARK7 antibody
Parkinson's disease locus DJ-1 (PARK7) is a differentially expressed transcript. DJ-1 plays a physiologic role in protection of erythroid cells from oxidant damage, a function unmasked in the context of oxidative stress. PARK7 belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Mutations in the DJ-1 gene are associated with rare forms of autosomal recessive early-onset Parkinson's disease (PD). DJ-1/p53 interactions contribute to apoptosis resistance in clonal myeloid cells and may serve as a prognostic marker in patients with myelodysplastic syndromes (MDS). DJ-1 regulates redox signaling kinase pathways and acts as a transcriptional regulator of antioxidative gene batteries. Therefore, DJ-1 is an important redox-reactive signaling intermediate controlling oxidative stress after ischemia, upon neuroinflammation, and during age-related neurodegenerative processes. Augmenting DJ-1 activity might provide novel approaches to treating chronic neurodegenerative illnesses such as Parkinson's disease and acute damage such as stroke.
References
Takahashi K, et al. (2001). DJ-1 positively regulates the androgen receptor by impairing the binding of PIASx alpha to the receptor. J. Biol. Chem. (United States). 276 (40): 37556-63.Niki, Takeshi, et al. (2003). DJBP: a novel DJ-1-binding protein, negatively regulates the androgen receptor by recruiting histone deacetylase complex, and DJ-1 antagonizes this inhibition by abrogation of this complex. Mol. Cancer Res. (United States). 1 (4): 247-61.Kahle PJ, et al. (2009) DJ-1 and prevention of oxidative stress in Parkinson's disease and other age-related disorders. Free Radic Biol Med. 47(10): 1354-61.Xu X, et al. (2010) The familial Parkinson's disease gene DJ-1 (PARK7) is expressed in red cells and plays a role in protection against oxidative damage. Blood Cells Mol Dis. 45(3): 227-32.Marcondes AM, et al. (2010) Identification of DJ-1/PARK-7 as a determinant of stroma-dependent and TNF-alpha-induced apoptosis in MDS using mass spectrometry and phosphopeptide analysis. Blood. 115(10): 1993-2002.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,891 Da
NCBI Official Full Name
protein DJ-1
NCBI Official Synonym Full Names
parkinson protein 7
NCBI Official Symbol
PARK7
NCBI Official Synonym Symbols
DJ1; DJ-1
NCBI Protein Information
protein DJ-1; oncogene DJ1; Parkinson disease (autosomal recessive, early onset) 7
UniProt Protein Name
Protein DJ-1
UniProt Gene Name
PARK7
UniProt Entry Name
PARK7_HUMAN
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Product Notes
The PARK7 park7 (Catalog #AAA255584) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-PARK7/DJ-1 Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PARK7/DJ-1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the PARK7 park7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARK7/DJ-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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