WB (Western Blot)
(Lane 1:293T Cell Lysate, Lane 2: Hela Cell Lysate, Lane 3: MCF-7 Cell Lysate, Lane 4:M231 Cell Lysate, Lane 5: Jurkat Cell Lysate)
Mouse Paxillin Monoclonal Antibody | anti-PXN antibody
Paxillin antibody
Applications
Immunocytochemistry, Western Blot
Synonyms
Paxillin, Antibody; Paxillin antibody; Mouse monoclonal Paxillin antibody; Monoclonal Paxillin antibody; Anti-Paxillin antibody; PXN antibody; Anti Paxillin antibody; anti-PXN antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
PAX-14
Specificity
Monoclonal PXN antibody specific for Human PXN.
Form/Format
Lyophilized powder
Sequence Length
591
Applicable Applications for anti-PXN antibody
ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Paxillin antibody was raised in mouse using C-terminal part of recombinant chicken paxillin (amino acids 305-559) as the immunogen
Post-Translational Modifications
PXN is Phosphorylated by MAPK1/ERK2. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion.
Preparation and Storage
Store at 4 degree C for short term storage.
For long term storage aliquot and store at-20 degree C.
Avoid repeated freeze/thaw cycles.
For long term storage aliquot and store at-20 degree C.
Avoid repeated freeze/thaw cycles.
WB (Western Blot)
(Lane 1:293T Cell Lysate, Lane 2: Hela Cell Lysate, Lane 3: MCF-7 Cell Lysate, Lane 4:M231 Cell Lysate, Lane 5: Jurkat Cell Lysate)
WB (Western Blot)
(Lane 1:293T Cell Lysate, Lane 2: Hela Cell Lysate, Lane 3: MCF-7 Cell Lysate, Lane 4:M231 Cell Lysate, Lane 5: Jurkat Cell Lysate)
Application Data
Application Data
Related Product Information for anti-PXN antibody
Biological Significance: Paxillin (PXN) is a signal transduction adaptor protein. The C-terminal region of paxillin contains four LIM domains that target paxillin to focal adhesions. It is presumed through a direct association with the cytoplasmic tail of beta-integrin. The N-terminal region of paxillin is rich in protein protein interaction sites. The proteins that bind to paxillin are diverse and include protein tyrosine kinases, such as Src and focal adhesion kinase (FAK), structural proteins, such as vinculin and actopaxin, and regulators of actin organization, such as COOL/PIX and PKL/GIT.
Sequence Info: PXN is composed of 591 amino acids with a molecular weight of 64505 Da. There are 5 LD motifs at positions 3 15, 144 156, 216 228, 265 276 and 333 345 and 4 LIM zinc-binding domains at 356 415, 416 473, 474 533 and 534 591.
Implications in Disease: Paxillin and cortactin have been shown to colocalize at invadopodia and play a pivotal role in breast cancer invasion. Similar colocalization of PXN has been observed in corectal cancer.
Expression Note: Elevated PXN expression has been reported in several human carcinomas in particular breast cancer. PXN is found mostly in the cytoplasm and is concentrated at focal adhesion junctions.
Sequence Info: PXN is composed of 591 amino acids with a molecular weight of 64505 Da. There are 5 LD motifs at positions 3 15, 144 156, 216 228, 265 276 and 333 345 and 4 LIM zinc-binding domains at 356 415, 416 473, 474 533 and 534 591.
Implications in Disease: Paxillin and cortactin have been shown to colocalize at invadopodia and play a pivotal role in breast cancer invasion. Similar colocalization of PXN has been observed in corectal cancer.
Expression Note: Elevated PXN expression has been reported in several human carcinomas in particular breast cancer. PXN is found mostly in the cytoplasm and is concentrated at focal adhesion junctions.
Product Categories/Family for anti-PXN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
46,604 Da
NCBI Official Full Name
Paxillin
NCBI Official Synonym Full Names
paxillin
NCBI Official Symbol
PXN
NCBI Protein Information
paxillin
UniProt Protein Name
Paxillin
UniProt Gene Name
PXN
Similar Products
Product Notes
The PXN pxn (Catalog #AAA224986) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Paxillin can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PXN pxn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Paxillin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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