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WB (Western Blot)
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WB (Western Blot) (Detection of mouse PBRM1 by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3, EL4, mIMCD-3, RenCa, NIH 3T3, TCMK-1, C2C12, A20, Ba/F3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

PBRM1 recombinant antibody

Rabbit anti-PBRM1 Recombinant Monoclonal Antibody [BL-39-2C3]

Gene Names
PBRM1; PB1; BAF180
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
PBRM1; Monoclonal Recombinant Antibody; Rabbit anti-PBRM1 Recombinant Monoclonal Antibody [BL-39-2C3]; BAF180; protein polybromo-1; polybromo-1D; PB1; BRG1-associated factor 180; hPB1; Protein polybromo-1; polybromo 1; PBRM1 recombinant antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BL-39-2C3
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for PBRM1 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS), Simple Western Size (SW-Size)
Application Notes
WB: 1:1000
IP: 5 ul/mg lysate
IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ICC: 1:100-1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
FC/FACS: Fixed and permeabilized cells were stained with 0.8 ul per 1 x 10^6 cells.
Immunogen
Residues 1-293
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse PBRM1 by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3, EL4, mIMCD-3, RenCa, NIH 3T3, TCMK-1, C2C12, A20, Ba/F3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot) (Detection of mouse PBRM1 by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3, EL4, mIMCD-3, RenCa, NIH 3T3, TCMK-1, C2C12, A20, Ba/F3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot)

(Detection of human PBRM1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot) (Detection of human PBRM1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

Simple Western

(Detection of mouse PBRM1 by Simple Western. Samples: Whole cell lysate (0.08 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.)

Simple Western (Detection of mouse PBRM1 by Simple Western. Samples: Whole cell lysate (0.08 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.)

IP (Immunoprecipitation)

(Detection of human PBRM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 lot 4) used for IP at 5 ul/mg lysate. PBRM1 was also immunoprecipitated by a previous lot of this antibody (lot AAA23796-3). For blotting immunoprecipitated PBRM1, AAA23796 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IP (Immunoprecipitation) (Detection of human PBRM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 lot 4) used for IP at 5 ul/mg lysate. PBRM1 was also immunoprecipitated by a previous lot of this antibody (lot AAA23796-3). For blotting immunoprecipitated PBRM1, AAA23796 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry)

(Detection of human PBRM1 in FFPE breast by immunohistochemistry-IF. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 Lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI.)

IHC (Immunohistochemistry) (Detection of human PBRM1 in FFPE breast by immunohistochemistry-IF. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 Lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI.)

IHC (Immunohistochemistry)

(Detection of human PBRM1 in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of human PBRM1 in FFPE breast carcinoma by immunohistochemistry. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human PBRM1 in FFPE MCF7 cells by immunocytochemistry. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human PBRM1 in FFPE MCF7 cells by immunocytochemistry. Antibody: Rabbit anti-PBRM1 recombinant monoclonal antibody [BL-39-2C3] (AAA23796 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human PBRM1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-PBRM1 recombinant monoclonal [BL-39-2C3] (AAA23796 lot 4) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human PBRM1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-PBRM1 recombinant monoclonal [BL-39-2C3] (AAA23796 lot 4) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for PBRM1 recombinant antibody
The SMARCs (SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin), and BAFs (BRG1-associated factors), have been identified as components of the mammalian SWI/SNF-like chromatin-remodeling protein complexes. These multi-protein complexes are proposed to function as ATP-driven motors that translocate along DNA and destabilize nucleosomal structures to facilitate transcription factor binding. PBRM1 is a component of the SWI/SNF-B (PBAF) complex. It contains six bromodomains which are defined by a 110 amino-acid module that interacts with acetylated lysine and is found in many chromatin binding proteins and histone acetyltransferases. PBRM1 has been proposed to function as a tumor suppressor protein. Recently, PBRM1 has been found to play a role in mediating cell cycle arrest in response to DNA damage via the regulation of the tumor suppressor p21.
Product Categories/Family for PBRM1 recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
192,948 Da
NCBI Official Full Name
protein polybromo-1
NCBI Official Synonym Full Names
polybromo 1
NCBI Official Symbol
PBRM1
NCBI Official Synonym Symbols
PB1; BAF180
NCBI Protein Information
protein polybromo-1; polybromo-1D; BRG1-associated factor 180
UniProt Protein Name
Protein polybromo-1
UniProt Gene Name
PBRM1
UniProt Synonym Gene Names
BAF180; PB1; hPB1; BAF180
UniProt Entry Name
PB1_HUMAN

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Product Notes

The PBRM1 pbrm1 (Catalog #AAA23796) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PBRM1 Recombinant Monoclonal Antibody [BL-39-2C3] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PBRM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS), Simple Western Size (SW-Size). WB: 1:1000 IP: 5 ul/mg lysate IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ICC: 1:100-1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. IHC-IF: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. FC/FACS: Fixed and permeabilized cells were stained with 0.8 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the PBRM1 pbrm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PBRM1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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