FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with PCAF antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Rabbit PCAF Monoclonal Antibody | anti-PCAF antibody
PCAF Antibody
Gene Names
KAT2B; P; CAF; PCAF; P/CAF
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
Purity
ProA affinity purified
Synonyms
PCAF, Antibody; PCAF Antibody; CAF antibody; CREBBP associated factor antibody; GCN5 antibody; GCN5L antibody; GCN5L1 antibody; Histone acetylase PCAF antibody; Histone acetyltransferase KAT2B antibody; Histone acetyltransferase PCAF antibody; K (lysine) acetyltransferase 2B antibody; KAT2B antibody; KAT2B_HUMAN antibody; Lysine acetyltransferase 2B antibody; P antibody; P/CAF antibody; p300/CBP associated factor antibody; P300/CBP-associated factor antibody; Pcaf antibody; anti-PCAF antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JU88-63
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PCAF antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with PCAF antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with PCAF antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of PCAF on rat kidney tissue (1) and K562 cell (2) lysate using anti-PCAF antibody at 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of PCAF on rat kidney tissue (1) and K562 cell (2) lysate using anti-PCAF antibody at 1/500 dilution.)
Related Product Information for anti-PCAF antibody
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessiblity to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.
Product Categories/Family for anti-PCAF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
93,013 Da
NCBI Official Full Name
histone acetyltransferase KAT2B
NCBI Official Synonym Full Names
K(lysine) acetyltransferase 2B
NCBI Official Symbol
KAT2B
NCBI Official Synonym Symbols
P; CAF; PCAF; P/CAF
NCBI Protein Information
histone acetyltransferase KAT2B; histone acetylase PCAF; CREBBP-associated factor; p300/CBP-associated factor; lysine acetyltransferase 2B; histone acetyltransferase PCAF
UniProt Protein Name
Histone acetyltransferase KAT2B
UniProt Gene Name
KAT2B
UniProt Synonym Gene Names
PCAF; Histone acetylase PCAF; P/CAF
UniProt Entry Name
KAT2B_HUMAN
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Product Notes
The PCAF kat2b (Catalog #AAA312596) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PCAF Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PCAF can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PCAF kat2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCAF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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