IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Monoclonal Antibody (PCNA/694))
Mouse PCNA (Proliferating Cell Nuclear Antigen) Monoclonal Antibody | anti-PCNA antibody
PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Mouse Monoclonal Antibody
Gene Names
PCNA; ATLD2
Reactivity
Human.Shows broad species reactivity
Applications
Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
PCNA (Proliferating Cell Nuclear Antigen), Antibody; PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Mouse Monoclonal Antibody; Cyclin; DNA polymerase delta auxiliary protein; Mutagen-sensitive 209 protein; PCNAR; Polymerase delta accessory protein; anti-PCNA antibody
Host
Mouse
Reactivity
Human.
Shows broad species reactivity
Shows broad species reactivity
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
PCNA/694
Specificity
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
261
Applicable Applications for anti-PCNA antibody
IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Cellular Localization
Predominantly nuclear, some cytoplasmic
Immunogen
Recombinant full length human PCNA protein
Hu-Chromosome Location
20p12.3
Positive Control
Tonsil or reactive lymph node
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Monoclonal Antibody (PCNA/694))
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Monoclonal Antibody (PCNA/694))
WB (Western Blot)
(Western Blot Analysis of HepG2 Cell Lysate using PCNA Monoclonal Antibody (PCNA/694))
WB (Western Blot)
(Western Blot Analysis of HepG2 Cell Lysate using PCNA Monoclonal Antibody (PCNA/694))
References
Waseem NH & Lane DP. 1990. J Cell Sci. 96:121-9. Hall PA et al. 1990. J. Pathol. 162(4):285-94
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36kDa
NCBI Official Full Name
proliferating cell nuclear antigen
NCBI Official Synonym Full Names
proliferating cell nuclear antigen
NCBI Official Symbol
PCNA
NCBI Official Synonym Symbols
ATLD2
NCBI Protein Information
proliferating cell nuclear antigen
UniProt Protein Name
Proliferating cell nuclear antigen
UniProt Gene Name
PCNA
UniProt Synonym Gene Names
PCNA
UniProt Entry Name
PCNA_HUMAN
Similar Products
Product Notes
The PCNA pcna (Catalog #AAA62806) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Mouse Monoclonal Antibody reacts with Human. Shows broad species reactivity and may cross-react with other species as described in the data sheet. AAA Biotech's PCNA (Proliferating Cell Nuclear Antigen) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the PCNA pcna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCNA (Proliferating Cell Nuclear Antigen), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.