Mouse PCSK1 / PC1 Monoclonal Antibody | anti-PCSK1 antibody

Mouse Monoclonal [clone 3D2] (IgG3,k) to Human PCSK1 / PC1

Cat. Num. AAA51894

• Gene Names: PCSK1; PC1; PC3; NEC1; SPC3; BMIQ12
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Purified fromascites by Protein A
• Synonyms: PCSK1 / PC1, Antibody; Mouse Monoclonal [clone 3D2] (IgG3,k) to Human PCSK1 / PC1; Anti-PCSK1 / PC1 Antibody (clone 3D2) IHC-plus; PCSK1; Pc1/3; PC1; Prohormone convertase 3; SPC3; Neuroendocrine convertase 1; PC3; Prohormone convertase 1; BMIQ12; NEC 1; NEC1; Proprotein convertase 1; Human PCSK1; anti-PCSK1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG3,k
• Clone Number: 3D2
• Specificity: Human PCSK1
• Purity/Purification: Purified fromascites by Protein A
• Form/Format: PBS, pH 7.4.
• Concentration: 0.48 mg/ml (varies by lot)
• Sequence Length: 753

• Applicable Applications for anti-PCSK1 antibody: ELISA, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
• Family/Subfamily: Protease/Serine S8
• Immunogen Species: Human
• Antigen Type: Recombinant Protein
• Immunogen: PCSK1 (NP_000430, 652 a.a. ~ 754 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: PCSK1 / PC1 antibody was raised against pCSK1 (NP_000430, 652 a.a. ~ 754 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20 degree C

ELISA

(Detection limit for recombinant GST tagged PCSK1 is approximately 0.03 ng/ml as a capture antibody.)

WB (Western Blot)

(PCSK1 monoclonal antibody clone 3D2. Western blot of PCSK1 expression in PC-12.)

WB (Western Blot)

(PCSK1 monoclonal antibody clone 3D2. Western blot of PCSK1 expression in NIH/3T3.)

IF (Immunofluorescence)

(Immunofluorescence of monoclonal antibody to PCSK1 on HeLa cell. [antibody concentration 30 ug/ml])

IHC (Immunohistochemistry)

(Anti-PCSK1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-PCSK1 antibody

PCSK1 is a Serine S8 type protease.

Product Categories/Family for anti-PCSK1 antibody

Family: Protease; Subfamily: Serine S8

NCBI and Uniprot Product Information

• NCBI GI #: 20336242
• NCBI GeneID: 5122
• NCBI Accession #: NP_000430.3
• NCBI GenBank Nucleotide #: NM_000439.4
• UniProt Accession #: P29120; P78478; Q92532; B7Z8T7; E9PHA1
• Molecular Weight: 79,010 Da
• NCBI Official Full Name: neuroendocrine convertase 1 isoform 1 preproprotein
• NCBI Official Synonym Full Names: proprotein convertase subtilisin/kexin type 1
• NCBI Official Symbol: PCSK1
• NCBI Official Synonym Symbols: PC1; PC3; NEC1; SPC3; BMIQ12
• NCBI Protein Information: neuroendocrine convertase 1; prohormone convertase 1; prohormone convertase 3
• UniProt Protein Name: Neuroendocrine convertase 1
• UniProt Gene Name: PCSK1
• UniProt Synonym Gene Names: NEC1; NEC 1; PC1
• UniProt Entry Name: NEC1_HUMAN