IF (Immunofluorescence)
(Immunofluorescence staining of Hela Cells at 1?50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG ?H+L?.)
PCYT1A recombinant antibody
PCYT1A Antibody
Gene Names
PCYT1A; CT; CTA; CCTA; CTPCT; PCYT1
Reactivity
Human
Applications
Immunofluorescence, Western Blot, ELISA
Purity
Affinity-chromatography
Synonyms
PCYT1A, Recombinant Antibody; PCYT1A Antibody; Choline-phosphate cytidylyltransferase A (EC 2.7.7.15) (CCT-alpha) (CTP:phosphocholine cytidylyltransferase A) (CCT A) (CT A) (Phosphorylcholine transferase A); PCYT1A; CTPCT PCYT1; PCYT1A recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
4F2
Purity/Purification
Affinity-chromatography
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for PCYT1A recombinant antibody
IF (Immunofluorescence), WB (Western Blot), ELISA
Immunogen
A synthesized peptide derived from human PCYT1A
Immunogen Species
Homo sapiens (Human)
Research Area
Neuroscience; Cancer: Metabolism: Signal Transduction
Target Names
PCYT1A
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IF (Immunofluorescence)
(Immunofluorescence staining of Hela Cells at 1?50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG ?H+L?.)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela Cells at 1?50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG ?H+L?.)
WB (Western Blot)
(Western BlotPositive WB detected in: K562 whole cell lysateAll lanes: PCYT1A antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42 kDaObserved band size: 42 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: K562 whole cell lysateAll lanes: PCYT1A antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42 kDaObserved band size: 42 kDa)
Related Product Information for PCYT1A recombinant antibody
In yeast, fly, and mammalian cells, PCYT1A, the rate-limiting enzyme of phosphatidylcholine (PC) synthesis, is intranuclear and re-locates to the nuclear membrane in response to the demand for membrane PL production. Membrane lipid stored curvature elastic (SCE) stress is increased by PC deficiency. PCYT1A deletions caused functional impairment in cells that generate PC for secretion as well as membrane maintenance. Lipodystrophy, spondylometaphyseal dysplasia with cone-rod dystrophy (SMD-CRD), and isolated retinal dystrophy are all caused by mutations in the PCYT1A gene. The preparation of the recombinant PCYT1A antibody involves the mammalian cell lines expression of plasma vectors containing PCYT1A antibody genes. B cells isolated from immunized animals' blood were treated to obtain RNA, which underwent reverse transcription to yield DNA genes. Antibody genes were sequenced and screened from the DNA. After transient expression, cell supernatant was collected and then purified using Affinity-chromatography to obtain the recombinant PCYT1A antibody. This recombinant PCYT1A antibody is recommended to use in the PCYT1A for the detection of PCYT1A protein from Human.
Product Categories/Family for PCYT1A recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
CTP:phosphocholine cytidylyltransferase
NCBI Official Synonym Full Names
phosphate cytidylyltransferase 1, choline, alpha
NCBI Official Symbol
PCYT1A
NCBI Official Synonym Symbols
CT; CTA; CCTA; CTPCT; PCYT1
NCBI Protein Information
choline-phosphate cytidylyltransferase A; CT A; CCT A; CCT-alpha; phosphorylcholine transferase A; CTP:phosphocholine cytidylyltransferase A
UniProt Protein Name
Choline-phosphate cytidylyltransferase A
UniProt Gene Name
PCYT1A
UniProt Synonym Gene Names
CTPCT; PCYT1; CCT A; CT A
UniProt Entry Name
PCY1A_HUMAN
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Product Notes
The PCYT1A pcyt1a (Catalog #AAA243836) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PCYT1A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCYT1A can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PCYT1A pcyt1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCYT1A, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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