Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Have a question or looking for a specific datasheet Manual/COA/MSDS?
Submit a Question

Interested to get a quote about our products?
Request a Quote

ELISA
>
<
ELISA (Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA abs at decreasing concentrations.)

Mouse anti-Human PD-1 Monoclonal Antibody | anti-PDCD1 antibody

PD-1 Antibody [8A4]

Gene Names
PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A purified IgG1.
Synonyms
PD-1; Monoclonal Antibody; PD-1 Antibody [8A4]; PD-1 Antibody; PD1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; Programmed cell death protein 1; Protein PD-1; PDCD1; PDCD-1; anti-PDCD1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8A4
Purity/Purification
Protein A purified IgG1.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
288
Applicable Applications for anti-PDCD1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Western blot at 0.5 - 1 mug/mL
Immunohistochemistry starting at 5 mug/mL.
Conjugate
Unconjugated
Immunogen
PD-1 antibody was raised against the extracellular domain of human PD-1.
Buffer
PD-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Species
Human
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles
Antibodies should not be exposed to prolonged high temperatures.

ELISA

(Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA abs at decreasing concentrations.)

ELISA (Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA abs at decreasing concentrations.)

WB (Western Blot)

(Western blot analysis of PD-1 in overexpressing 293 cells using antibody at 1, 0.5, and 0.25 μg/ml, respectively.)

WB (Western Blot) (Western blot analysis of PD-1 in overexpressing 293 cells using antibody at 1, 0.5, and 0.25 μg/ml, respectively.)

IF (Immunofluorescence)

(Immunofluorescence of PD-1 in transfected 293 cells with PD-1 antibody at 5 μg/mL. Lower left: Immunofluorescence in transfected 293 cells with control mouse IgG antibody at 5 μg/mL.Red: PD1 Antibody [8A4]Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of PD-1 in transfected 293 cells with PD-1 antibody at 5 μg/mL. Lower left: Immunofluorescence in transfected 293 cells with control mouse IgG antibody at 5 μg/mL.Red: PD1 Antibody [8A4]Blue: DAPI staining)

ICC (Immunocytochemistry)

(Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 5 μg/mL.)

ICC (Immunocytochemistry) (Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 5 μg/mL.)

IHC (Immunohistochemistry)

(Immunohistochemistry of PD-1 in (A) human breast cancer tissue and (B) human normal breast tissue with PD-1 antibody at 5 μg/mL.)

IHC (Immunohistochemistry) (Immunohistochemistry of PD-1 in (A) human breast cancer tissue and (B) human normal breast tissue with PD-1 antibody at 5 μg/mL.)

IHC (Immunohistochemistry)

(Immunohistochemistry of PD-1 in (A) human tonsil tissue, (B) human lymph node tissue, and (C) human spleen tissue with PD-1 antibody at 5 μg/mL. (D) Immunohistochemistry in human tonsil tissue with control mouse IgG staining at 5 μg/mL.)

IHC (Immunohistochemistry) (Immunohistochemistry of PD-1 in (A) human tonsil tissue, (B) human lymph node tissue, and (C) human spleen tissue with PD-1 antibody at 5 μg/mL. (D) Immunohistochemistry in human tonsil tissue with control mouse IgG staining at 5 μg/mL.)

WB (Western Blot)

(Western blot analysis of PD-1 in transfected 293 cell lysate with PD-1 antibody at (A) 0.5 and (B) 1 μg/mL.)

WB (Western Blot) (Western blot analysis of PD-1 in transfected 293 cell lysate with PD-1 antibody at (A) 0.5 and (B) 1 μg/mL.)
Related Product Information for anti-PDCD1 antibody
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD-1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. PD-1 is thus one of a growing number of immune checkpoint proteins.
Product Categories/Family for anti-PDCD1 antibody
References
1. Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90. 2. Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95. 3. Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8. 4. Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 1999; 11:141-51.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Predicted: 32 kDa
Observed: 38 kDa
NCBI Official Full Name
Programmed cell death protein 1
NCBI Official Synonym Full Names
programmed cell death 1
NCBI Official Symbol
PDCD1
NCBI Official Synonym Symbols
PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
NCBI Protein Information
programmed cell death protein 1
UniProt Protein Name
Programmed cell death protein 1
UniProt Gene Name
PDCD1
UniProt Synonym Gene Names
PD1; Protein PD-1; hPD-1

Similar Products

PD-1 Antibody

Host: Rabbit
Reactivity: Human, Mouse, Rat
Apps: Western Blot, Immunohistochemistry, Immunofluorescence

PDCD1 Antibody

Host: Mouse
Reactivity: Human
Apps: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay

PDCD1 Antibody

Host: Mouse
Reactivity: Human
Apps: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

PDCD1 ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a

PDCD1 ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a

PDCD1 ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a

PDCD1 Antibody

Host: Mouse
Reactivity: Human
Apps: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay

PD-L1 Antibody

Host: Mouse
Reactivity: Human
Apps: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay

PD-ECGF Antibody

Host: Rabbit
Reactivity: Human
Apps: Western Blot, Immunohistochemistry

PD-L2 Antibody

Host: Mouse
Reactivity: Human
Apps: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation

CD279/PDCD1/PD1 Recombinant Protein

Host: Mammalian cells
Reactivity: n/a
Apps: SDS-Page, Western Blot, ELISA

ICAM-1 Antibody

Host: Rabbit
Reactivity: Human, Mouse, Monkey
Apps: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA

IGF-1 ELISA Kit

Host: n/a
Reactivity: Rat
Apps: n/a

KAP-1 Antibody

Host: Mouse
Reactivity: Human, Mouse, Rat
Apps: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot

MMP-1 ELISA Kit

Host: n/a
Reactivity: Rat
Apps: n/a

KOR-1 Antibody

Host: Rabbit
Reactivity: Mouse, Rat
Apps: Western Blot, Immunohistochemistry

MCP-1 ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a

GLP-1 ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a

TSST 1 ELISA Kit

Host: n/a
Reactivity: Human
Apps: n/a

ET-1 ELISA Kit

Host: n/a
Reactivity: Mouse
Apps: n/a

Product Notes

The PDCD1 pdcd1 (Catalog #AAA10979) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PD-1 Antibody [8A4] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PD-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF). Western blot at 0.5 - 1 mug/mL Immunohistochemistry starting at 5 mug/mL.Precautions All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

aaabiotech.com © 2012-2025

All rights reserved.

Contact us by

Tel: +1 (619) 604-9440 | Fax: +1 (619) 604-9440
[email protected]

Submit a Question

Please complete the form below and a representative will contact you as soon as possible.

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.