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Application Data
(Biotinylated Human PD-1/PDCD1 Protein SPR DataHuman PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1, His-Avi Tag with an affinity constant of 2.356 ?M as determined in SPR assay (Biacore T200).)
PD-1/PDCD1 recombinant protein
Biotinylated Human PD-1/PDCD1 Protein Recombinant
Gene Names
PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
PD-1/PDCD1; N/A; Biotinylated Human PD-1/PDCD1 Protein Recombinant; PD-1/PDCD1 recombinant protein
Host
HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Human PD-1/PDCD1 was lyophilized from 0.2 um filtered PBS solution pH7.4
Source
DNA sequence encoding Human PD-1/PDCD1 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Biological Process
Immunity
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at-20 degree C.
Application Data
(Biotinylated Human PD-1/PDCD1 Protein SPR DataHuman PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1, His-Avi Tag with an affinity constant of 2.356 ?M as determined in SPR assay (Biacore T200).)
Application Data
(Biotinylated Human PD-1/PDCD1 Protein SPR DataHuman PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human PD-1, His-Avi Tag with an affinity constant of 2.356 ?M as determined in SPR assay (Biacore T200).)
ELISA
(Biotinylated Human PD-1/PDCD1 Protein ELISA DataImmobilized Biotinylated Human PD-1, His Tag at 0.5?g/ml (100?l/Well) on the plate. Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.)
ELISA
(Biotinylated Human PD-1/PDCD1 Protein ELISA DataImmobilized Biotinylated Human PD-1, His Tag at 0.5?g/ml (100?l/Well) on the plate. Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA.)
Application Data
(Biotinylated Human PD-1/PDCD1 Protein SEC-HPLCThe purity of Biotinylated Human PD-1 is greater than 95% as determined by SEC-HPLC.)
Application Data
(Biotinylated Human PD-1/PDCD1 Protein SEC-HPLCThe purity of Biotinylated Human PD-1 is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE
(Biotinylated Human PD-1/PDCD1 Protein Tris-Bis PAGEBiotinylated Human PD-1 on Tris-Bis PAGE under reduced. The purity is greater than 95%.)
Tris-Bis-PAGE
(Biotinylated Human PD-1/PDCD1 Protein Tris-Bis PAGEBiotinylated Human PD-1 on Tris-Bis PAGE under reduced. The purity is greater than 95%.)
Product Categories/Family for PD-1/PDCD1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
31,647 Da
NCBI Official Full Name
Programmed cell death protein 1
NCBI Official Synonym Full Names
programmed cell death 1
NCBI Official Symbol
PDCD1
NCBI Official Synonym Symbols
PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
NCBI Protein Information
programmed cell death protein 1
UniProt Protein Name
Programmed cell death protein 1
UniProt Gene Name
PDCD1
UniProt Synonym Gene Names
PD1; Protein PD-1; hPD-1
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Product Notes
The PD-1/PDCD1 pdcd1 (Catalog #AAA76292) is a Recombinant Protein produced from HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PD-1/PDCD1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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