IP (Immunoprecipitation)
(Immunoprecipitating PD-L2 in Hela whole cell lysate Lane 1: Mouse control IgG instead of in Hela whole cell lysate. Lane 2: (2ul) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (20ug) For western blotting, the blot was detected at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 )
Mouse anti-Human PD-L2 Monoclonal Antibody | anti-PD-L2 antibody
PD-L2 Monoclonal Antibody
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH7.4
IHC: 1:50-1:200
IF: 1:50-1:200
FC/FACS: 1:50-1:200
IP:2ul-5ul
IP (Immunoprecipitation)
(Immunoprecipitating PD-L2 in Hela whole cell lysate Lane 1: Mouse control IgG instead of in Hela whole cell lysate. Lane 2: (2ul) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (20ug) For western blotting, the blot was detected at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 )
IP (Immunoprecipitation)
(Immunoprecipitating PD-L2 in Hela whole cell lysate Lane 1: Mouse control IgG instead of in Hela whole cell lysate. Lane 2: (2ul) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (20ug) For western blotting, the blot was detected at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 )
FCM (Flow Cytometry)
(Overlay histogram showing 293 cells transfected with PD-L2 stained with (red line). The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (2ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was R-PE-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (2ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM (Flow Cytometry)
(Overlay histogram showing 293 cells transfected with PD-L2 stained with (red line). The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (2ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was R-PE-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (2ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IF (Immunofluorescence)
(Immunofluorescence staining of U251 cells at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of U251 cells at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Raji cells at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Raji cells at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cells at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cells at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IHC (Immunohistochemistry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry)
(IHC image diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot)
(Western Blot Positive WB detected in: Hela whole cell lysate at 20ug, 10ug, 5ug, 2.5ug, 1.25ug All lanes: PD-L2 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 31,21 KDa Observed band size: 45-50 KDa Exposure time?5min)
WB (Western Blot)
(Western Blot Positive WB detected in: Hela whole cell lysate at 20ug, 10ug, 5ug, 2.5ug, 1.25ug All lanes: PD-L2 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 31,21 KDa Observed band size: 45-50 KDa Exposure time?5min)
WB (Western Blot)
(Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate All lanes PD-L2 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 31,21 KDa Observed band size: 45-50 KDa Exposure time?5min)
WB (Western Blot)
(Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate All lanes PD-L2 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 31,21 KDa Observed band size: 45-50 KDa Exposure time?5min)
NCBI and Uniprot Product Information
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Product Notes
The PD-L2 pdcd1lg2 (Catalog #AAA27047) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PD-L2 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PD-L2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:5000-1:640,000 IHC: 1:50-1:200 IF: 1:50-1:200 FC/FACS: 1:50-1:200 IP:2ul-5ul. Researchers should empirically determine the suitability of the PD-L2 pdcd1lg2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PD-L2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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