IHC (Immunohiostchemistry)
(Paraffin embedded sections of human breast lobule tissue were incubated with anti-human PDCD12 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
Mouse anti-Human, Mouse PDCD12 Monoclonal Antibody | anti-PDCD12 antibody
PDCD12 antibody
Gene Names
AVEN; PDCD12
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
By protein-G affinity chromatography
Synonyms
PDCD12, Antibody; PDCD12 antibody; cell death regulator aven; apoptosis; caspase activation inhibitor; PDCD12; programmed cell death 12; anti-PDCD12 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
3G4
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
362
Applicable Applications for anti-PDCD12 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human PDCD12 (254-362aa) purified from E. coli
Clone Note
Anti-human PDCD12 mAb, clone 3G4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PDCD12 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IHC (Immunohiostchemistry)
(Paraffin embedded sections of human breast lobule tissue were incubated with anti-human PDCD12 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
IHC (Immunohiostchemistry)
(Paraffin embedded sections of human breast lobule tissue were incubated with anti-human PDCD12 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
WB (Western Blot)
(The extracts of mouse kidney (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD12 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot)
(The extracts of mouse kidney (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDCD12 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-PDCD12 antibody
PDCD12 (Programmed cell death 12), also referred to as AVEN, represents a new class of cell death regulator. PDCD12 is a conserved intracellular membrane protein that blocks apoptosis impeded by Apaf-1 mediated caspase activation. Bcl-2 and BAD also interact with PDCD12 to prevent apoptosis. It is expressed in a wide variety of normal tissues including heart, skeletal muscle, kidney, liver, testis, and diseases such as cancer
Product Categories/Family for anti-PDCD12 antibody
References
Figueroa B Jr, et al: (2004) Biotechnol Bioeng. 85 (6), 589-600; Cheng EH, et al: (2000) Mol Cell. 6 (1), 31-40
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,506 Da
NCBI Official Full Name
cell death regulator Aven
NCBI Official Synonym Full Names
apoptosis and caspase activation inhibitor
NCBI Official Symbol
AVEN
NCBI Official Synonym Symbols
PDCD12
NCBI Protein Information
cell death regulator Aven
UniProt Protein Name
Cell death regulator Aven
UniProt Gene Name
AVEN
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Product Notes
The PDCD12 aven (Catalog #AAA48034) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PDCD12 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PDCD12 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PDCD12 aven for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDCD12, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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