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WB (Western Blot)
(Detection of mouse PDGFR alpha by western blot. Samples: Whole cell lysate (50 ug) from mIMCD-3, A20, NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PDGFR alpha recombinant monoclonal antibody (AAA213594 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
PDGFR alpha Recombinant Antibody | PDGFRalpha recombinant antibody
Rabbit anti-PDGFR alpha Recombinant Monoclonal Antibody [BLR128H]
Gene Names
PDGFRA; CD140A; PDGFR2; PDGFR-2; RHEPDGFRA
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
PDGFR alpha, Recombinant Antibody; Rabbit anti-PDGFR alpha Recombinant Monoclonal Antibody [BLR128H]; platelet-derived growth factor receptor alpha; PDGFR-2; platelet-derived growth factor receptor 2; Platelet-derived growth factor alpha receptor; PDGFR-alpha; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; CD140a antigen; CD140A; CD140 antigen-like family member A; CD antigen CD140a; alpha-type platelet-derived growth factor receptor; Alpha platelet-derived growth factor receptor; PDGFR2; Platelet-derived growth factor receptor alpha; platelet derived growth factor receptor alpha; PDGFRalpha recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR128H
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for PDGFRalpha recombinant antibody
ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Residues 24-525 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse PDGFR alpha by western blot. Samples: Whole cell lysate (50 ug) from mIMCD-3, A20, NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PDGFR alpha recombinant monoclonal antibody (AAA213594 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of mouse PDGFR alpha by western blot. Samples: Whole cell lysate (50 ug) from mIMCD-3, A20, NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PDGFR alpha recombinant monoclonal antibody (AAA213594 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human PDGFR alpha by western blot. Samples: Whole cell lysate (50 ug) from NCI-H1703, HeLa, IMR-90, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PDGFR alpha recombinant monoclonal antibody (AAA213594 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human PDGFR alpha by western blot. Samples: Whole cell lysate (50 ug) from NCI-H1703, HeLa, IMR-90, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PDGFR alpha recombinant monoclonal antibody (AAA213594 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human PDGFR alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 5% of IP loaded) from NCI-H1703 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PDGFR alpha recombinant monoclonal antibody (AAA213594 Lot 1) used for IP at 20 ul/mg lysate. PDGFR alpha was also immunoprecipitated by rabbit anti-PDGFR alpha antibody BL-1170-1G6. For blotting immunoprecipitated PDGFR alpha, AAA213594 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human PDGFR alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 5% of IP loaded) from NCI-H1703 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-PDGFR alpha recombinant monoclonal antibody (AAA213594 Lot 1) used for IP at 20 ul/mg lysate. PDGFR alpha was also immunoprecipitated by rabbit anti-PDGFR alpha antibody BL-1170-1G6. For blotting immunoprecipitated PDGFR alpha, AAA213594 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)
ICC (Immunocytochemistry)
(Detection of human PDGFR alpha in FFPE NCI-H1703 cells by immunocytochemistry. Antibody: Rabbit anti-PDGFR alpha recombinant monoclonal antibody (AAA213594 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human PDGFR alpha in FFPE NCI-H1703 cells by immunocytochemistry. Antibody: Rabbit anti-PDGFR alpha recombinant monoclonal antibody (AAA213594 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of mouse PDGFR alpha in FFPE 3T3 cells by immunocytochemistry. Antibody: Rabbit anti-PDGFR alpha recombinant monoclonal antibody (AAA213594 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of mouse PDGFR alpha in FFPE 3T3 cells by immunocytochemistry. Antibody: Rabbit anti-PDGFR alpha recombinant monoclonal antibody (AAA213594 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for PDGFRalpha recombinant antibody
Platelet derived growth factor receptor alpha is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR alpha and beta polypeptides. PDGFR alpha plays a role in organ development, wound healing, and tumor progression [taken from NCBI Entrez Gene (Gene ID: 5156)].
Product Categories/Family for PDGFRalpha recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
122,670 Da
NCBI Official Full Name
platelet-derived growth factor receptor alpha
NCBI Official Synonym Full Names
platelet-derived growth factor receptor, alpha polypeptide
NCBI Official Symbol
PDGFRA
NCBI Official Synonym Symbols
CD140A; PDGFR2; PDGFR-2; RHEPDGFRA
NCBI Protein Information
platelet-derived growth factor receptor alpha; PDGF-R-alpha; CD140a antigen; PDGFRA/BCR fusion; CD140 antigen-like family member A; platelet-derived growth factor receptor 2; alpha-type platelet-derived growth factor receptor; rearranged-in-hypereosinophi
UniProt Protein Name
Platelet-derived growth factor receptor alpha
UniProt Gene Name
PDGFRA
UniProt Synonym Gene Names
PDGFR2; RHEPDGFRA; PDGF-R-alpha; PDGFR-alpha; PDGFR-2
UniProt Entry Name
PGFRA_HUMAN
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Product Notes
The PDGFRalpha pdgfra (Catalog #AAA213594) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PDGFR alpha Recombinant Monoclonal Antibody [BLR128H] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PDGFR alpha can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PDGFRalpha pdgfra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDGFR alpha, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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