WB (Western Blot)
(Detection of mouse PDGFR beta by western blot. Samples: Whole cell lysate (10 ug) from A20, NIH 3T3, C6, C2C12, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213658 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
PDGFR beta Recombinant Antibody | PDGFRbeta recombinant antibody
Rabbit anti-PDGFR beta Recombinant Monoclonal Antibody [BLR242L]
Gene Names
G3BP1; G3BP; HDH-VIII
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
PDGFR beta, Recombinant Antibody; Rabbit anti-PDGFR beta Recombinant Monoclonal Antibody [BLR242L]; CD antigen CD140b; platelet-derived growth factor receptor beta variant 1; platelet-derived growth factor receptor beta; platelet-derived growth factor receptor 1; PDGFR-beta; PDGF-R-beta; PDGFR-1; Pdgfr; PDGF beta chain; AI528809; CD140 antigen-like family member B; beta-type platelet-derived growth factor receptor; beta platelet-derived growth factor receptor; CD140b; Platelet-derived growth factor receptor beta; platelet derived growth factor receptor, beta polypeptide; PDGFRbeta recombinant antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Clone Number
BLR242L
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for PDGFRbeta recombinant antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Residues 32-530 (ECD)
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse PDGFR beta by western blot. Samples: Whole cell lysate (10 ug) from A20, NIH 3T3, C6, C2C12, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213658 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of mouse PDGFR beta by western blot. Samples: Whole cell lysate (10 ug) from A20, NIH 3T3, C6, C2C12, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213658 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
ICC (Immunocytochemistry)
(Detection of mouse PDGFR beta by immunocytochemistry. Sample: FFPE section of mouse C2C12 cells. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213658 lot 1) used for IP at 6 ul/mg lysate. PDGFR beta was also immunoprecipitated by a second antibody against a different epitope of PDGFR beta (Antibody 2). For blotting immunoprecipitated PDGFR beta, AAA213658 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
ICC (Immunocytochemistry)
(Detection of mouse PDGFR beta by immunocytochemistry. Sample: FFPE section of mouse C2C12 cells. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213658 lot 1) used for IP at 6 ul/mg lysate. PDGFR beta was also immunoprecipitated by a second antibody against a different epitope of PDGFR beta (Antibody 2). For blotting immunoprecipitated PDGFR beta, AAA213658 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
FCM/FACS (Flow Cytometry)
(Detection of mouse PDGFR beta (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213658) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of mouse PDGFR beta (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-PDGFR beta recombinant monoclonal antibody (AAA213658) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for PDGFRbeta recombinant antibody
Platelet derived growth factor receptor beta is a cell surface tyrosine kinase receptor for homodimeric PDGFB and PDGFD and for heterodimers formed by PDGFA and PDGFB. These growth factors are members of the platelet-derived growth factor family and are mitogens for cells of mesenchymal origin. PDGFR beta is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. [taken from NCBI Entrez Gene (Gene ID: 5159)].
Product Categories/Family for PDGFRbeta recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,164 Da
NCBI Official Full Name
ras GTPase-activating protein-binding protein 1
NCBI Official Synonym Full Names
GTPase activating protein (SH3 domain) binding protein 1
NCBI Official Symbol
G3BP1
NCBI Official Synonym Symbols
G3BP; HDH-VIII
NCBI Protein Information
ras GTPase-activating protein-binding protein 1; G3BP-1; GAP binding protein; ATP-dependent DNA helicase VIII; GAP SH3 domain-binding protein 1; RasGAP-associated endoribonuclease G3BP; Ras-GTPase-activating protein SH3-domain-binding protein
UniProt Protein Name
Platelet-derived growth factor receptor beta
UniProt Gene Name
Pdgfrb
UniProt Synonym Gene Names
Pdgfr; Pdgfr1; PDGF-R-beta; PDGFR-beta; PDGFR-1
UniProt Entry Name
PGFRB_MOUSE
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Product Notes
The PDGFRbeta pdgfrb (Catalog #AAA213658) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-PDGFR beta Recombinant Monoclonal Antibody [BLR242L] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PDGFR beta can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the PDGFRbeta pdgfrb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDGFR beta, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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