IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using PDH-E1? Monoclonal Antibody.)
Mouse PDH-E1alpha Monoclonal Antibody | anti-PDH-E1alpha antibody
PDH-E1alpha Monoclonal Antibody
Gene Names
PDHA1; PDHA; PHE1A; PDHCE1A
Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Affinity purification
Synonyms
PDH-E1alpha, Antibody; PDH-E1alpha Monoclonal Antibody; Pyruvate dehydrogenase E1 component subunit alpha somatic form mitochondrial; PDHA1; PHE1A; Pyruvate dehydrogenase E1 component subunit alpha; somatic form, mitochondrial; PDHE1-A type I; anti-PDH-E1alpha antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig
Clonality
Monoclonal
Specificity
PDH-E1alpha Monoclonal Antibody detects endogenous levels of PDH-E1alpha protein.
Purity/Purification
Affinity purification
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PDH-E1alpha antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Purified recombinant human PDH-E1? (C-terminus) protein fragments expressed in E coli.
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using PDH-E1? Monoclonal Antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using PDH-E1? Monoclonal Antibody.)
WB (Western Blot)
(Western Blot analysis using PDH-E1? Monoclonal Antibody against A549. HeLa cell lysate.)
WB (Western Blot)
(Western Blot analysis using PDH-E1? Monoclonal Antibody against A549. HeLa cell lysate.)
Related Product Information for anti-PDH-E1alpha antibody
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components; pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits.This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]
Product Categories/Family for anti-PDH-E1alpha antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,296 Da
NCBI Official Full Name
pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial isoform 1
NCBI Official Synonym Full Names
pyruvate dehydrogenase (lipoamide) alpha 1
NCBI Official Symbol
PDHA1
NCBI Official Synonym Symbols
PDHA; PHE1A; PDHCE1A
NCBI Protein Information
pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; pyruvate dehydrogenase complex, E1-alpha polypeptide 1
UniProt Protein Name
Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial
UniProt Gene Name
PDHA1
UniProt Synonym Gene Names
PHE1A
UniProt Entry Name
ODPA_HUMAN
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Product Notes
The PDH-E1alpha pdha1 (Catalog #AAA320273) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PDH-E1alpha Monoclonal Antibody reacts with Human, Mouse, Rat, Bovine, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's PDH-E1alpha can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the PDH-E1alpha pdha1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDH-E1alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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