FCM (Flow Cytometry)

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FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with PDK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

Rabbit PDK2 Monoclonal Antibody | anti-PDK2 antibody

PDK2 Antibody

Gene Names

PDK2; PDHK2; PDKII

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation

Purity

ProA affinity purified

Synonyms

PDK2; Monoclonal Antibody; PDK2 Antibody; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 antibody; mitochondrial antibody; PDHK2 antibody; PDK2 antibody; PDK2_HUMAN antibody; Pyruvate dehydrogenase kinase isoform 2 antibody; Pyruvate dehydrogenase kinase; isozyme 2 antibody; Pyruvate dehydrogenase lipoamide kinase isozyme 2; anti-PDK2 antibody

Ordering
Cat. Num. AAA30453 Quantity For Research Use Only!

Host

Rabbit

Reactivity

Human, Mouse, Rat

Clonality

Monoclonal

Clone Number

JB66-93

Purity/Purification

ProA affinity purified

Form/Format

1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Applicable Applications for anti-PDK2 antibody

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)

Application Notes

WB: 1:500
IHC: 1:50-1:200
FC/FACS: 1:50-1:100

Antibody Type

Recombinant Antibody

Immunogen

Recombinant protein

Conjugation

Unconjugated

Preparation and Storage

Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of SH-SY-5Y cells with PDK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-PDK2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-PDK2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PDK2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PDK2 antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of PDK2 on rat heart tissue lysate using anti-PDK2 antibody at 1/500 dilution.)

Related Product Information for anti-PDK2 antibody

Pyruvate dehydrogenase kinase family members (PDK1, 2, 3, 4) are serine kinases that catalyze the phosphorylation of the E1alpha subunit of the pyruvate dehydrogenase complex (PDC). PDC activity is controlled through phosphorylation and dephosphorylation of the E1alpha subunit, which leads to inactivation and reactivation, respectively. The core of PDC is composed of sixty dihydrolypoyl acetyltransferase (E2) subunits that bind directly to PDK2 and enhance PDK2 kinase activity. Upregulation of PDK isoenzymes occurs during starvation conditions, rerouting acetyl-CoA generation by facilitating fatty acid oxidation. PDKs contain five conserved regions and are mechanistically similar to bacterial His-kinases, in that both require Histidine residues for activity. In mammals, transcripts for PDK2 are ubiquitously expressed with high levels in heart and skeletal muscle and decreased levels in spleen and lung.

Product Categories/Family for anti-PDK2 antibody

Total protein Ab

NCBI and Uniprot Product Information

NCBI GI #

315630349

NCBI GeneID

5164

NCBI Accession #

NP_001186827.1

NCBI GenBank Nucleotide #

NM_001199898.1

UniProt Accession #

Q15119; Q6P515; Q9BS05; A8K3A7; B3KNW0

Molecular Weight

46,154 Da

NCBI Official Full Name

pyruvate dehydrogenase kinase, isozyme 2 isoform 2

NCBI Official Synonym Full Names

pyruvate dehydrogenase kinase, isozyme 2

NCBI Official Symbol

PDK2

NCBI Official Synonym Symbols

PDHK2; PDKII

NCBI Protein Information

pyruvate dehydrogenase kinase, isozyme 2; PDH kinase 2; pyruvate dehydrogenase kinase, isoenzyme 2; pyruvate dehydrogenase, lipoamide, kinase isozyme 2, mitochondrial

UniProt Protein Name

[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial

UniProt Gene Name

PDK2

UniProt Synonym Gene Names

PDHK2; PDH kinase 2; PDKII

UniProt Entry Name

PDK2_HUMAN

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PDHK2 [Pyruvate Dehydrogenase [Lipoamide]] Kinase Isozyme 2, Mitochondrial, Pyruvate Dehydrogenase Kinase Isoform 2, PDH Kinase 2, PDKII, PDK2) Antibody

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Apps: ELISA, Western Blot, Immunohistochemistry

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Product Notes

The PDK2 pdk2 (Catalog #AAA30453) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDK2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PDK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:500 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the PDK2 pdk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDK2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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