FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing THP-1 cells stained with AAA279157 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
PECAM1 recombinant antibody
PECAM1 Recombinant Monoclonal Antibody
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
Affinity purified
Synonyms
PECAM1, Recombinant Antibody; PECAM1 Recombinant Monoclonal Antibody; Adhesion molecule; CD31; CD31 antigen; CD31 EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA; GPIIA'; PECA1; PECA1_HUMAN; Pecam 1; PECAM 1 CD31 EndoCAM; PECAM; PECAM-1; Pecam1; Platelet and endothelial cell adhesion molecule 1; Platelet endothelial cell adhesion molecule; Platelet/endothelial cell adhesion molecule 1; PECAM1 recombinant antibody
Host
HEK293F Cell
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
9C9
Purity/Purification
Affinity purified
Form/Format
Liquid; 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
Applicable Applications for PECAM1 recombinant antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
Recombinant Human PECAM1 protein
Conjugation
Non-conjugated
Research Area
Cancer; Cardiovascular; Immunology; Signal transduction; Stem cells
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing THP-1 cells stained with AAA279157 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing THP-1 cells stained with AAA279157 (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
WB (Western Blot)
(Western BlotPositive WB detected in: THP-1 whole cell lysate, JK whole cell lysateAll lanes: PECAM1 antibody at 1:500SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 82-140 kDaObserved band size: 140 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: THP-1 whole cell lysate, JK whole cell lysateAll lanes: PECAM1 antibody at 1:500SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 82-140 kDaObserved band size: 140 kDa)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
82,536 Da
NCBI Official Full Name
platelet endothelial cell adhesion molecule
NCBI Official Synonym Full Names
platelet/endothelial cell adhesion molecule 1
NCBI Official Symbol
PECAM1
NCBI Official Synonym Symbols
CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
NCBI Protein Information
platelet endothelial cell adhesion molecule; CD31 antigen
UniProt Protein Name
Platelet endothelial cell adhesion molecule
UniProt Gene Name
PECAM1
UniProt Synonym Gene Names
PECAM-1
UniProt Entry Name
PECA1_HUMAN
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Product Notes
The PECAM1 pecam1 (Catalog #AAA279157) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The PECAM1 Recombinant Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PECAM1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PECAM1 pecam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PECAM1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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