SDS-PAGE
(SDS-PAGE Analysis of Purified Perforin-1 mouse Monoclonal Antibody (PRF-2468). Confirmation of Purity and Integrity of Antibody.)
Mouse Perforin-1 Monoclonal Antibody | anti-PRF1 antibody
Perforin-1 (Pore Forming Protein) (Apoptosis Marker)
Gene Names
PRF1; P1; PFP; HPLH2
Reactivity
Human. Others not known.
Applications
Immunohistochemistry
Synonyms
Perforin-1, Antibody; Perforin-1 (Pore Forming Protein) (Apoptosis Marker); Cytolysin; FLH2; HPLH2; Lymphocyte pore-forming protein; PRF1 (pore forming protein 1); Perforin-1; PFP; PGFL; PIGF; PIGF-2; PLGF; anti-PRF1 antibody
Host
Mouse
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
PRF1/2468
Specificity
Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
555
Applicable Applications for anti-PRF1 antibody
IHC (Immunohistochemistry)
Immunogen
Recombinant human Perforin-1 protein fragment (around aa 413-552)
Cellular Localization
Cytoplasmic
Hu-chromosome Location
10q22.1
Positive Control
HDLM-2 Cells. Spleen
Preparation and Storage
Antibody with azide store at 2-8°C.
Antibody is stable for 24 months. Non hazardous. No msds requiresd.
Antibody is stable for 24 months. Non hazardous. No msds requiresd.
SDS-PAGE
(SDS-PAGE Analysis of Purified Perforin-1 mouse Monoclonal Antibody (PRF-2468). Confirmation of Purity and Integrity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis of Purified Perforin-1 mouse Monoclonal Antibody (PRF-2468). Confirmation of Purity and Integrity of Antibody.)
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Perforin-1 Mouse Monoclonal Antibody (PRF1/2468).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Perforin-1 Mouse Monoclonal Antibody (PRF1/2468).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Spleen stained with Perforin-1 Mouse Monoclonal Antibody (PRF1/2468).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Spleen stained with Perforin-1 Mouse Monoclonal Antibody (PRF1/2468).)
References
Bittmann I, et al. Fas/FasL and perforin/granzyme pathway in acute rejection and diffuse alveolar damage after allogeneic lung transplantation- a human biopsy study. Virchows Arch. 2004; 445:375-81.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75kDa
NCBI Official Full Name
perforin-1
NCBI Official Synonym Full Names
perforin 1
NCBI Official Symbol
PRF1
NCBI Official Synonym Symbols
P1; PFP; HPLH2
NCBI Protein Information
perforin-1
UniProt Protein Name
Perforin-1
UniProt Gene Name
PRF1
UniProt Synonym Gene Names
PFP; P1; PFP
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Product Notes
The PRF1 prf1 (Catalog #AAA214681) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Perforin-1 (Pore Forming Protein) (Apoptosis Marker) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's Perforin-1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the PRF1 prf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Perforin-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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