Application Data
(Figure A. FITC conjugated Mouse anti Human CD56 and RPE conjugated mouse IgG2b isotype control . Figure B. FITC conjugated Mouse Anti Human CD56 and RPE conjugated Mouse anti Human Perforin . All experiments were performed on human peripheral blood lymphocytes blocked with 10% human serum, fixed and permeabilized. Gated on live, single cell lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
Mouse anti-Human Perforin Monoclonal Antibody | anti-PRF1 antibody
Mouse Anti Human Perforin
Gene Names
PRF1; P1; PFP; HPLH2
Reactivity
Human
Applications
Immunoprecipitation, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
>95% by SDS PAGEPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Synonyms
Perforin, Antibody; Mouse Anti Human Perforin; Perforin antibody, dG9; anti-PRF1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
dG9
Purity/Purification
>95% by SDS PAGE
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Form/Format
Purified IgG - Liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
555
Applicable Applications for anti-PRF1 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Immunogen
Purified granules from human YT lymphoma cell line
Preservative Stabilizers
<0.1% Sodium Azide (NaN3)
Buffer Solution
TRIS Buffered Saline
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE.
This product should be stored undiluted.
This product should be stored undiluted.
Application Data
(Figure A. FITC conjugated Mouse anti Human CD56 and RPE conjugated mouse IgG2b isotype control . Figure B. FITC conjugated Mouse Anti Human CD56 and RPE conjugated Mouse anti Human Perforin . All experiments were performed on human peripheral blood lymphocytes blocked with 10% human serum, fixed and permeabilized. Gated on live, single cell lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
Application Data
(Figure A. FITC conjugated Mouse anti Human CD56 and RPE conjugated mouse IgG2b isotype control . Figure B. FITC conjugated Mouse Anti Human CD56 and RPE conjugated Mouse anti Human Perforin . All experiments were performed on human peripheral blood lymphocytes blocked with 10% human serum, fixed and permeabilized. Gated on live, single cell lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
Application Data
(Intracellular taining of perforin in human peripheral blood cells with Mouse anti Human perforin:RPE)
Application Data
(Intracellular taining of perforin in human peripheral blood cells with Mouse anti Human perforin:RPE)
Related Product Information for anti-PRF1 antibody
Mouse anti Human perforin, clone dG9 recognizes perforin a 60-70 kDa glycoprotein largely expressed in CD8+ cytotoxin T lymphocytes and Natural Killer cells. It aids in defense against virus-infected or neoplastic cells by inducing apoptosis. Perforin achieves this by inserting a portion of its C-terminus region into the lipid membrane of the target in a calcium dependent manner. Subsequent polymerization with other perforin molecules forms a pore in the membrane. These pores allow the free influx and efflux of ions and proteins though the cell membrane. This disruption to homeostasis results in tonic shock which can ultimately lead to apoptosis and DNA degradation (Osinska et al. 2014).
Genetic abnormalities to the perforin gene have been shown to be linked to abnormal immune system functioning, the development of immune diseases as well as lymphomas and leukemias (Osinska et al. 2014).
This clone dG9 has been used in flow cytometry experiments to examine intracellular perforin in tumour specific CD8+ T cells incubated with autologous primary breast cancer cells and Tregs (Su et al. 2017).
Genetic abnormalities to the perforin gene have been shown to be linked to abnormal immune system functioning, the development of immune diseases as well as lymphomas and leukemias (Osinska et al. 2014).
This clone dG9 has been used in flow cytometry experiments to examine intracellular perforin in tumour specific CD8+ T cells incubated with autologous primary breast cancer cells and Tregs (Su et al. 2017).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
perforin-1
NCBI Official Synonym Full Names
perforin 1
NCBI Official Symbol
PRF1
NCBI Official Synonym Symbols
P1; PFP; HPLH2
NCBI Protein Information
perforin-1
UniProt Protein Name
Perforin-1
UniProt Gene Name
PRF1
UniProt Synonym Gene Names
PFP; P1; PFP
UniProt Entry Name
PERF_HUMAN
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Product Notes
The PRF1 prf1 (Catalog #AAA50390) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human Perforin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Perforin can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the PRF1 prf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Perforin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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