IF (Immunofluorescence)
(ICC/IF analysis of PGD in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGD antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human PGD Monoclonal Antibody | anti-PGD antibody
PGD antibody
Gene Names
PGD; 6PGD
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
PGD, Antibody; PGD antibody; Phosphogluconate dehydrogenase; 6PGD; Phosphogluconate dehydrogenase 0610042A05Rik; Au019875; C78335; 6 phosphogluconate dehydrogenase; decarboxylating; OTTMuSP00000011754; RP23-249G3.4,; anti-PGD antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT46B3
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
483
Applicable Applications for anti-PGD antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human PGD (1-483aa) purified from E. coli
Preparation and Storage
Can be stored at +2°C to +8°C for up to 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of PGD in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGD antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of PGD in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGD antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGD antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: MCF7 cell lysate Lane 3.: 293T cell lysate Lane 4.: A549 cell lysate Lane 5.: Jurkat cell lysate)
WB (Western Blot)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGD antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: MCF7 cell lysate Lane 3.: 293T cell lysate Lane 4.: A549 cell lysate Lane 5.: Jurkat cell lysate)
Related Product Information for anti-PGD antibody
PGD (Phosphogluconate dehydrogenas), also known as 6PGD, is a 483 amino acid enzyme that is involved in the pentose phosphate shunt. Pentose is required for nucleic acid biosynthesis and the pentose phosphate cycle is a major source of NADPH. PGD deficiency increases the level of erythrocyte pyruvate kinase (PK) activity and reduces glutathione synthetase (GSH), resulting in hemolysis. Defects in PGD are generally asymptomatic and are inherited in an autosomal dominant fashion.
Product Categories/Family for anti-PGD antibody
References
Le TD., et al. (2010) Proc Natl Acad Sci U S A. 107 (7): 3198-203.; Tagen M., et al. (2009) J Immunol. 183 (10): 6313-9;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
6-phosphogluconate dehydrogenase, decarboxylating isoform 1
NCBI Official Synonym Full Names
phosphogluconate dehydrogenase
NCBI Official Symbol
PGD
NCBI Official Synonym Symbols
6PGD
NCBI Protein Information
6-phosphogluconate dehydrogenase, decarboxylating
UniProt Protein Name
6-phosphogluconate dehydrogenase, decarboxylating
UniProt Gene Name
PGD
UniProt Synonym Gene Names
PGDH
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Product Notes
The PGD pgd (Catalog #AAA48230) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PGD antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PGD can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the PGD pgd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGD, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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